下调IKKα表达能够抑制胃癌细胞的迁移和侵袭能力  

Downregulation of IKKα inhibits gastric cancer cells migration and invasion

作　　者：尚鑫[1] 刘剑[1] 张琳[1] 夏丽敏[1] 吴开春[1] 

机构地区：[1]第四军医大学西京消化病医院,肿瘤生物学国家重点实验室,陕西西安710032

出　　处：《现代肿瘤医学》2014年第5期977-980,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172290;81272652;91129723)

摘　　要：目的:研究降低IKKα的表达对人胃癌细胞迁移和侵袭能力的影响。方法:利用Western blot检测胃癌细胞系中IKKα蛋白的表达水平,选择IKKα表达较高的细胞系MKN28,转染下调IKKα的慢病毒,建立稳定转染细胞株(标记为MKN28-LV-shcontrol和MKN28-LV-shIKKα),并利用Western blot、RT-PCR验证病毒转染效果。采用Transwell实验和划痕实验检测低表达IKKα对胃癌细胞迁移和侵袭能力的影响。结果:Western blot结果显示胃癌细胞系中MKN28细胞IKKα蛋白表达水平相对较高,转染慢病毒干扰载体成功后利用Western blot及RT-PCR实验证实实验组MKN28-LV-shIKKα较对照组MKN28-LV-shcontrol中IKKα蛋白及mRNA水平明显降低。利用Transwell及划痕实验证实实验组MKN28-LV-shIKKα较对照组MKN28-LV-shcontrol细胞迁移及侵袭能力明显降低。结论:IKKα与胃癌的迁移及侵袭密切相关,降低IKKα的表达水平能显著抑制胃癌细胞的迁移和侵袭能力,提示IKKα在胃癌的转移中可能发挥着重要作用。Objective：To exPlore the migration and invasion ability of gastric cancer cells after silencing IKKα ex-Pression. Methods：We used Western blot to detect the IKKα exPression in different gastric cancer cells,and then transfected IKKα shRNA lentivirus and control lentivirus into MKN - 28 cells exPressing high level of IKKα. Western blot and RT - PCR were Performed to verified the silencing effect. And then we conducted transwell migration and in-vasion assay and the wound - healing assay to deliberate the role of IKKα in GC metastasis. Results：Western blot showed that the exPression level of IKKα in MKN28 cells were higher than other gastric cancer cell lines. After trans-fection of IKKα shRNA,the Protein and mRNA level of IKKα in the MKN28 - LV - shIKKα was significantly de-creased. The migration and invasion ability of MKN28 - LV - shIKKα was significantly decreased comPared with MKN28 - LV - shcontrol. Conclusion：Silencing of IKKα significantly decreased the invasion and migration ability of gastric cancer cells. Targeting IKKα may therefore Provide a Promising theraPeutic strategy for gastric cancer Patients.

关 键 词：胃癌 IKKα 侵袭 转移 

分 类 号：R73-362[医药卫生—肿瘤] R735.2[医药卫生—临床医学]