ING4诱导甲状腺癌SW579细胞S早期阻滞的机制研究  

ING4-induced S phase early stage arrest in thyroid cancer cells SW579 and its mechanism

作　　者：王传疆[1] 王雷[1] 胡海地[1] 

机构地区：[1]中国医科大学附属第一医院血管甲状腺外科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2014年第5期1001-1004,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81000136)

摘　　要：目的:检测ING4对甲状腺癌SW579细胞周期的影响及其机制。方法:利用软琼脂克隆形成实验检测细胞增殖,并利用PI染色和Cldu/Idu双染检测细胞周期变化。Western blot技术检测周期相关蛋白的变化。结果:ING4可以明显抑制SW579细胞增殖,并且导致其S早期阻滞。ING4处理后细胞p53表达明显升高,同时phospho-S345-Chk1和phospho-T68-Chk2等细胞周期相关蛋白表达水平升高。结论:ING4可导致SW579细胞出现S早期阻滞,p53表达明显升高,细胞周期相关蛋白表达水平升高,但是三者关系有待进一步研究。Objective：To study the effects and mechanisms of ING4 on cell cycle of thyroid cancer cell SW579. Methods：The Proliferative ratio of SW579 cells after treated with ING4 was detected by soft agarose assay. We detec-ted the changes of cell cycle after treated with ING4 by PI staining and Cldu/ Idu double staining. The cell cycle relat-ed Proteins were analyzed by Western blot. Results：ING4 could inhibit the Proliferation and induced S Phase early stage arrest in SW579 cells. The levels of cell cycle related Proteins,P53,PhosPho - S345 - Chk1 and PhosPho - T68- Chk2,were higher in treated cells than untreated ones. Conclusion：ING4 could induce S early stage arrest in SW579 cell,uP - regulate P53 exPression and cell cycle related Proteins. eowever,the relationshiP among ING4,P53 and cell cycle related Proteins by activating P53 exPression was needed further studies.

关 键 词：SW579细胞 ING4 S期 增殖 P53 

分 类 号：R730.23[医药卫生—肿瘤] R736.1[医药卫生—临床医学]