黄芪甲苷对肝癌细胞增殖和侵袭能力的影响及其机制研究  

The anti-proliferation and anti-invasion effect of astragaloside IV on liver cancer cell line in vitro and its mechanism

作　　者：马鹏飞[1] 阮柏[1] 王德盛[1] 窦科峰[1] 

机构地区：[1]第四军医大学西京医院肝胆胰脾外科,陕西西安710032

出　　处：《现代肿瘤医学》2014年第5期1012-1015,共4页Journal of Modern Oncology

基　　金：国家自然科学基金重点项目(编号:81030010/H0318);陕西省科技研究发展计划项目(编号:2012SF2-09-1)

摘　　要：目的:研究黄芪甲苷对肝癌细胞系MHCC97-H增殖和侵袭能力的影响。方法:MTT法检测5、10、20、40、80、100μg/ml不同终浓度和24、48、72h作用时间下黄芪甲苷对MHCC97-H细胞增殖的影响。Transwell侵袭实验检测100μg/ml黄芪甲苷处理48小时对MHCC97-H细胞侵袭能力的影响。选择100μg/ml黄芪甲苷处理48小时作为处理条件,实时定量PCR、Western blot检测黄芪甲苷处理后MHCC97-H细胞MMP-2、9 mRNA和蛋白质表达水平的变化,明胶酶谱法检测黄芪甲苷处理后MHCC97-H细胞培养上清中MMP-2、9分泌水平的变化。结果:各浓度和作用时间下黄芪甲苷对MHCC97-H细胞增殖均无明显抑制作用。100μg/ml黄芪甲苷处理48小时后,MHCC97-H细胞侵袭能力降低,MMP-2、9分子mRNA和蛋白质表达水平均下降,且细胞培养上清中分泌的MMP-2、9减少。结论:体外黄芪甲苷对MHCC97-H细胞增殖无明显抑制作用,但黄芪甲苷可以通过降低MHCC97-H细胞MMP-2、9分子的表达起到降低其细胞侵袭能力的作用。Objective：To investigate the anti - Proliferation and anti - invasion effect of astragaloside IV on liver cancer cell line MeCC97 - e in vitro. Methods：MeCC97 - e cells were exPosed to different concentrations of astra-galoside IV（A - IV）for 24h,48h and 72h resPectively. The Proliferation was detected by 4 - methyl - teerazolium （MTT）. Transwell assay was aPlied to detect the inhibitory effect of A - IV on MeCC97 - e cell invasion. MeCC97- e cells were treated by 100μg/ ml A - IV for 48 hours. Then the exPressive changes for matrix metalloProteinase -2,9（MMP - 2,9）mRNA and Protein in MeCC97 - e cells were detected by real - time PCR and Western bolt re-sPectively. The contents of MMP - 2,9 in culture suPematant of MeCC97 - e cells were measured by gelatin zymog-raPhy. Results：MTT showed that A - IV had no significantly inhibitoty effect on MeCC97 - e cells with any concen-trations and times. The result of Transwell assay showed that A - IV could inhibit the invasion of MeCC97 - e cells in vitro. The exPressions of MMP - 2,9 mRNA and Protein reduced after treatment by 100μg/ ml A - IV for 48 hours. And the contents of MMP - 2,9 in culture suPematant of MeCC97 - e cells reduced. Conclusion：A - IV has no ob-vious inhibitory effect on the Proliferation of MeCC97 - e cells,but a strong inhibitory action on the invasion of Me-CC97 - e cells in vitro.

关 键 词：黄芪甲苷 肝癌 MHCC97-H细胞 增殖 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]