小分子化合物O-565抑制胃癌细胞迁移侵袭及机制研究  

The effect and mechanism of O-565 inhibiting the migration and invasion in gastric cancer cells

作　　者：张健[1] 刘奇[1] 李瑞娟[2] 张红艳[1] 李丹妮[3] 李丰[1] 

机构地区：[1]中国医科大学基础医学院细胞生物教研室教育部医学细胞生物学重点实验室,辽宁沈阳110001 [2]沈阳药科大学制药工程学院,辽宁沈阳110016 [3]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2014年第5期1020-1023,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81230077;313714 24;81302127)

摘　　要：目的:研究小分子化合物O-565对人胃癌细胞BGC823、MKN-45增殖、迁移和侵袭能力的影响及其作用机制。方法:应用MTT检测不同浓度小分子化合物O-565对胃癌细胞BGC823和MKN-45增殖能力的影响。分别用0、20、40、60μmol/L浓度的化合物O-565处理BGC823和MKN-45两种胃癌细胞,应用Transwell小室检测其对上述两种细胞迁移侵袭能力的抑制作用,Western blot检测化合物对金属基质蛋白酶2(MMP-2)表达及丝切蛋白(Cofilin)磷酸化水平的影响。结果:化合物O-565对于BGC823和MKN-45两种胃癌细胞的增殖能力无明显影响,却能够以剂量依赖的方式抑制上述细胞的迁移和侵袭。Western blot结果显示O-565能够下调胃癌细胞BGC823中MMP-2的表达及Cofilin的磷酸化水平。结论:化合物O-565可能通过下调细胞中MMP-2的表达及Cofilin的磷酸化水平,抑制BGC823、MKN-45胃癌细胞的迁移和侵袭。Objective：To study the effect and mechanism of O-565 on the Proliferation,migration and invasion in BGC823 and MKN-45 cells. Methods：MTT was used to detect the effect of O-565 on the Proliferation of BGC823 and MKN-45 cells. The test of Transwell was used to check the migration and invasion of BGC 823 and MKN-45 cells with 0,20,40,60 μmol/ L O-565. Western blot assay was adoPted to identify the effect of O-565 on the ex-Pression of Matrix MetalloProteinase 2（MMP - 2）and PhosPhorylated Cofilin. Results：O-565 did not affect the Proliferation of BGC823 and MKN-45 cells. But it could significantly inhibit the migration and invasion ability of BGC823 and MKN-45 cells in a dose - dePendent manner. Furthermore,O-565 could obviously down - regulate the exPression levels of MMP - 2 and PhosPhorylated Cofilin. Conclusion：O-565 could inhibit the migration and inva-sion caPacity of BGC823 and MKN-45 cells by down - regulating the exPression levels of MMP - 2 and PhosPhoryla-ted Cofilin.

关 键 词：O-565 胃癌 侵袭 MMP-2 COFILIN 

分 类 号：R735.2[医药卫生—肿瘤]