整合素连接激酶在非小细胞肺癌中过度表达及促进肺癌细胞的侵袭和迁移  

Over-expression of integrin linked kinase in non-small cell lung cancer and promoting lung cancer cells invasion and migration

作　　者：赵明静[1] 刘朔[1] 韩冰[1] 李方治[1] 凌媛[1] 毛世涛[1] 王笑歌[1] 张旭华[1] 

机构地区：[1]中国医科大学附属第四医院呼吸内科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2014年第5期1053-1058,共6页Journal of Modern Oncology

基　　金：辽宁省科学技术计划项目(编号:2012020104-202);辽宁省自然科学基金项目(编号:20092097)

摘　　要：目的:探讨整合素连接激酶(ILK)在非小细胞肺癌(NSCLC)中的表达及在侵袭和迁移中的作用和相关分子机制。方法:免疫组化法检测ILK蛋白在NSCLC患者中的表达,细胞转染、siRNA干扰、细胞划痕试验、实时定量PCR、Western blot方法探讨ILK在肺癌A549细胞中的表达及分子机制。结果:ILK蛋白在原发性NSCLC组织中过度表达30.6%(33/108)并且和TNM分期(P=0.001)、淋巴结转移(P=0.033)相关。ILK在A549细胞中过度表达并且通过下调E-cadherin,上调波形蛋白、纤维连接蛋白、Snail、Slug导致上皮-间质转化(EMT)。此外,NF-κB抑制剂BAY 11-7028和小干扰靶RNA(siRNA)NF-p65可诱导E-cadherin的表达下调。结论:ILK在原发性NSCLC组织中高表达并与TNM分期和淋巴结转移相关,其促进肺癌细胞的侵袭和迁徙机制可能是经NF-κB信号通路诱导EMT所致。Objective：To detect the exPression of integrin - linked kinase（ILK）in non - small cell lung cancer （NSCLC）and investigate the molecular mechanism in invasion and migration. Methods：The exPression of ILK was determined by means of immunohistochemistry in Patients with NSCLC. The exPression and molecular mechanism of ILK in A549 cells were studied by cell transfection,siRNA interference,cell scratch test,real - time quantitative PCR and Western blot. Results：The exPression rate of ILK was 30. 6%（33 / 108）in Primary NSCLC tissue that was asso-ciated with TNM staging（P = 0. 001）and lymPh node metastasis（P = 0. 033）. ILK was over - exPressing in A549 cells and led to ePithelial mesenchymal transition（EMT）through down - regulating E - cadherin,uP - regulating vi-mentin,fibronectin,Snail and Slug. In addition,NF - κB inhibitor BAY 11 - 7028 and small interference target RNA （siRNA）NF - P65 could induce the down - regulation of E - cadherin. Conclusion：ILK was over - exPressed in NSCLC and associated with TNM staging and lymPh node metastasis. ILK Promotes lung cancer cell migration and in-vasion by inducing EMT through NF - κB signal Pathway.

关 键 词：肺肿瘤 ILK EMT 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]