不同保存介质对脐带间充质干细胞活性的影响  

Effects of different storage solutions on the viability of umbilical cord mesenchymal stem cells

作　　者：周楠[1] 陈彦[2] 雷鑫[1] 董艳婷[1] 徐文婧[1] 牛玉虎[1] 牛勃[3] 程牛亮[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西省太原市030001 [2]山西医科大学第二附属医院呼吸科,山西省太原市030001 [3]首都儿科研究所生物技术研究室,北京市100020

出　　处：《中国组织工程研究》2014年第14期2153-2160,共8页Chinese Journal of Tissue Engineering Research

摘　　要：背景:脐带间充质干细胞的临床应用面临移植前保存的问题,而临床保存介质对移植前细胞活性的影响直接关乎移植后疗效,目前却缺乏对临床介质保存效果的系统性研究。目的:评价临床常用保存介质对脐带间充质干细胞活性的影响。方法:在4℃与室温(25℃)条件下,将脐带间充质干细胞在临床常用保存液(0.9%氯化钠注射液,5%葡萄糖注射液和1%人血白蛋白溶液)中保存2,4,6 h,观察上述保存介质对脐带间充质干细胞活性、凋亡/死亡率、多向分化能力及DNA损伤的影响。结果与结论:无论在4℃还是室温条件下,细胞在临床常用保存介质中的活性均在短期内呈时间依赖性下降。此外,不同保存介质引起细胞死亡而非凋亡,且死亡率呈时间依赖性增加;经不同介质保存后,细胞出现DNA损伤,但其增殖与多向分化能力未受明显影响,提示细胞核DNA损伤为细胞活性下降的关键因素。BACKGROUND： Umbilical cord-mesenchymal stem cells （UC-MSCs） have been widely studied in clinics. Notably, UC-MSCs have to be properly preserved before clinical use and the effects of storage solution on viability of cells play critical roles in the effectiveness of cell-transplantation. However, there are few studies concerning this phenomenon. OBJECTIVE： To evaluate the effects of various clinical common solutions on the activity of UC-MSCs. METHODS： UC-MSCs were stored at 0,9% sodium chloride injection, 5% glucose injection and 1% human serum albumin respectively at 4 ~C and room temperature （25 ~C） for 2, 4 or 6 hours. Viabilities, apoptosis/necrosis rate multi-differentiation ability of preserved UC-MSCs and DNA damage were examined. RESULTS AND CONCLUSION： The viabilities of UC-MSCs were rapidly decreased time-dependently in all of the storage solutions both at 4 ~C and room temperature. Besides, the storage solutions resulted in cellular necrosis rather than apoptosis and the necrosis rate was greatly increased time-dependently. DNA damage was observed in cells after preserved in various storage solutions, but cell proliferation and differentiation were not affected, which demonstrated that DNA damage may be a key factor for the reduction of cell viabilities.

关 键 词：脐带间充质干细胞 临床保存 细胞活性 介质 细胞核DNA损伤 临床应用 时间依赖性 氯化钠注射液 

分 类 号：R394.2[医药卫生—医学遗传学]