前列腺素E1对大鼠骨髓间充质干细胞增殖的影响  

Prostaglandin E1 effects on proliferation of rat bone marrow mesenchymal stem cells

作　　者：曾宽[1] 江慧琦[1] 王萌[1] 华平[1] 张洪武[2] 邓保平[1] 张书铭[1] 杨艳旗[1] 

机构地区：[1]中山大学孙逸仙纪念医院心脏外科,广东省广州市510120 [2]中山大学附属第一医院转化医学中心,广东省广州市510080

出　　处：《中国组织工程研究》2014年第14期2161-2166,共6页Chinese Journal of Tissue Engineering Research

基　　金：中山大学百人计划专项基金(F002009011)~~

摘　　要：背景:目前还缺乏前列腺素E1对骨髓间充质干细胞增殖影响的研究。目的:观察前列腺素E1与骨髓间充质干细胞共培养对细胞增殖作用的影响。方法:体外培养SD大鼠骨髓间充质干细胞。取第3代细胞利用流式细胞仪鉴定细胞表面表型。以每孔1×104数量接种于96孔板,将前列腺素E1分别以0(对照组),1,2,5,10,20,40,100μg/L的浓度作用骨髓间充质干细胞72 h,以CCK-8法检测细胞增殖情况,观察最佳增殖浓度10%的前列腺素E1在不同时间(24,48,72 h)对骨髓间充质干细胞增殖的影响。结果与结论:P3代骨髓间充质干细胞表面阳性率CD11b/c为4.93%,CD29为99.93%,CD45为0.1%,CD90为99.58%,符合骨髓间充质干细胞特征。质量浓度为1,2,5,10,20,40,100μg/L的前列腺素E1均可以促进骨髓间充质干细胞在体外的增殖,以10μg/L的作用最强。10μg/L前列腺素E1在作用48 h后能显著促进大鼠骨髓间充质干细胞增殖(P<0.05),其作用呈一定程度的时间依赖性,72 h达最高。BACKGROUND： Currently, there are few studies about prostaglandin E1 on the proliferation of bone marrow mesenchymal stem cells. OBJECTIVE： To explore the effects of prostaglandin E1 on the proliferation of bone marrow mesenchymal stern cells. METHODS： Bone marrow mesenchymal stem cells isolated from Sprague Dawley rats were cultured in vitro. Passage 3 cells were selected to identify cell surface phenotypes by flow cytometry. Cells were seeded onto 96-well microtiter plates at a density of 1 xl 04 cells/well, and then treated with prostaglandin E1 at concentrations of O, 1, 2, 5, 10, 20, 40, 100 pg/L for 3 days. Cell counting kit-8 assay was applied to assess cellular proliferation, and detect the optimal proliferative concentration of prostaglandin E1 （10%） effects on the proliferation of bone marrow mesenchymai stem at 24, 48 and 72 hours. RESULTS AND CONCLUSION： The positive rates of passage 3 cells were 4.93% for CD11 b/c, 99.93% for CD29 0.1% for CD45, 99.58% for CD90. In vitro proliferation of bone marrow mesenchymal stem cells could be promoted by prostaglandin E1 at concentrations of 1, 2, 5, 10, 20, 40 and 100 pg/L, and the optimal concentration was 10 pg/L. The proliferation of bone marrow mesenchymal stem cells was promoted dramatically after intervention with 10 pg/L prostaglandin E1 for 48 hours （P 〈 0.05）, and peaked at 72 hours in a time-dependent manner.

关 键 词：骨髓间充质干细胞 前列腺素E1 干细胞增殖 SD大鼠 细胞增殖作用 细胞增殖情况 CCK-8法 质量浓度 

分 类 号：R394.2[医药卫生—医学遗传学]