胚胎及骨髓间充质干细胞增殖活性与成脂分化能力的比较  

Comparison of proliferative and adipogenic differentiation potential of human mesenchymal stem cells derived from fetal tissue and bone marrow

作　　者：李秀英[1] 白金萍[2] 仲苓芝[2] 李雪[3] 杨麒巍 刘颖男[1] 王轶敏[1,4] 

机构地区：[1]吉林大学中日联谊医院中心实验室,吉林省长春市130033 [2]吉林大学基础医学院病理学教研室,吉林省长春市130021 [3]延边大学药理教研室,吉林省延吉市133002 [4]吉林省中科生物工程有限公司,吉林省长春市130012

出　　处：《中国组织工程研究》2014年第14期2173-2178,共6页Chinese Journal of Tissue Engineering Research

基　　金：吉林省自然科学基金(20130101167JC);课题名称:几种组织间充质干细胞免疫原性比较及临床自身免疫性疾病的应用~~

摘　　要：背景:间充质干细胞最先在骨髓中发现,且骨髓间充质干细胞的相关研究也最多,但是骨髓间充质干细胞来源有限且含量极低。近年来,已经从人胚胎组织分离出间充质干细胞,其与骨髓间充质干细胞在生物学特性上有很多相似之处,但是二者的成脂诱导能力比较尚无明确报道。目的:比较人胚胎组织与骨髓来源间充质干细胞体外增殖能力及成脂诱导能力。方法:采用全骨髓法获得骨髓间充质干细胞,胶原酶消化法获得胚胎组织间充质干细胞。流式细胞术鉴定两种间充质干细胞表面分子标记。用细胞计数方法检测两种间充质干细胞增殖能力。分别向两种细胞培养体系中加入成脂诱导剂诱导14 d,经油红O染色后观察并计数,计算不同来源间充质干细胞的成脂诱导分化率。结果与结论:从两种不同组织中均可获取梭形贴壁的间充质干细胞,表面标记物CD44、CD73、CD90和CD105均呈阳性表达,CD14、CD34和CD45均为阴性表达;胚胎组织间充质干细胞的增殖能力明显强于骨髓间充质干细胞(P<0.01)。胚胎组织间充质干细胞和骨髓间充质干细胞经过成脂诱导液诱导14 d后,油红O染色结果均为阳性,但是骨髓间充质干细胞体外成脂能力明显强于胚胎组织间充质干细胞(P<0.01)。BACKGROUND： Mesenchymal stem cells were first discovered in the bone marrow, and the number of studies concerning bone marrow mesenchymal stem cells is also the most. But the bone marrow mesenchymal stem cells with limited sources have the very low content. In recent years, mesenchymal stem cells have been isolated from human fetal tissue, which are similar to bone marrow mesenchymal stem cells in term of biological properties, but both of them have not been reported clearly in comparison of their adipogenic abilities. OBJECTIVE： To compare the proliferative and adipogenic differentiation capacity of human bone marrow and fetal tissue-derive mesenchymal stem cells. METHODS： Mesenchymal stem cells were obtained from the bone marrow by adherence culture and from fetal tissue by collagenase digestion. Cells were identified by flow cytometric assay. Proliferative potential of mesenchymal stem cells were detected by cell counting method. After adipogenic induction for 14 days, oil red O staining was used for cell counting, and adipogenic differentiation capacities of mesenchymal stem cell from two sources were measured.RESULTS AND CONCLUSION： Mesenchymal stem cells in spindle shape were successfully harvested from both of human bone marrow and fetal tissue, which were positive for CD73, CD90 and CD105, and negative for CD14, CD34 and CD45. The proliferative capacity of fetal tissue-derived mesenchymal stem ceils was stronger than that of bone marrow mesenchymal stem cells （P 〈 0.01）. After adipogenic induction for 14 days, mesenchymal stem cells from two sources were both positive for oil red O, but the in vitro adipogenic capacity of the bone marrow mesenchymal stern cells was stronger than that of fetal tissue-mesenchymal stem cells （P 〈 0.01）.

关 键 词：骨髓间充质干细胞 细胞增殖活性 胚胎组织 分化能力 成脂 细胞增殖能力 油红O染色 胶原酶消化法 

分 类 号：R394.2[医药卫生—医学遗传学]