成人眼小梁网内皮细胞具有干细胞/祖细胞的属性  

The stem/progenitor cell characteristics of trabecular meshwork endothelia cells in the adult human eye

作　　者：万尚韬 李永平[1] 张平[1] 高欢欢[1] 张文忻[1] 林健贤[1] 

机构地区：[1]中山大学中山眼科中心,眼科学国家重点实验室,眼病理科,广东省广州市510060

出　　处：《中国组织工程研究》2014年第14期2213-2218,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:干细胞/祖细胞的研究为干细胞治疗疾病提供了可能,寻找正常人眼内小梁网干细胞/祖细胞,探究小梁网干细胞的属性能为原发性开角型青光眼的干细胞/祖细胞治疗提供新的思路。目的:探索小梁网内皮干细胞/祖细胞的属性。方法:选取成人眼球20例,其中10例眼球标本来源于因急性外伤大失血死亡后摘除的眼球,眶内容物剜除的眼球7例,3例来源于眼后段黑色素瘤患者手术摘除眼球。所有标本行苏木精-伊红染色以及免疫组化染色观察。结果与结论:小梁网内皮细胞中阳性表达nestin,阴性表达凝血因子Ⅷ,CD31和CD34,而角膜内皮细胞中弱阳性表达nestin,Schlemm管内皮细胞未见nestin阳性表达,但凝血因子Ⅷ,CD31,CD34均呈强阳性表达。表明小梁网内皮细胞均具有干细胞/祖细胞的属性,小梁网nestin蛋白阳性的内皮细胞相似于表达nestin的血管内皮祖细胞。BACKGROUND： The study of stem/progenitor cells provides the possibility of stem cell therapy, and identification and characterization of stem/progenitor cells in human trabecuiar meshwork can put new thoughts on treatment of primary open-angle glaucoma. OBJECTIVE： To study the characteristics of stem/progenitor cells in trabecular meshwork in the adult human eye. METHODS： Hematoxylin and eosin staining and immunohistochemistry staining of nestin, Factor VIII-related antigen, CD31 and CD34 were applied in 20 enucleated adult human eyes were selected, among which, 10 normal eyes had enucleation due to sudden death for serious trauma, 7 eyes were from the patients who had orbital exenteration, and 3 eyes were diagnosed as posterior segment choroidal malignant melanoma. RESULTS AND CONCLUSION： The trabecular meshwork endothelial cells in the human eye were all positive for nestin, but negative for the other three anti-bodies. The corneal endothelial cells were weakly positive for nestin. Schlemm＇s canal endothelial cells were negative for nestin, but strongly positive for the Factor VIII-related antigen CD31 and CD34. These findings indicate that the trabecular meshwork endothelial cells in the human eye all express nestin with stem/progenitor cell characteristics, and they are similar to the vascular progenitor cells with nestin expression.

关 键 词：小梁网内皮细胞 血管内皮祖细胞 干细胞治疗 nestin蛋白 原发性开角型青光眼 摘除眼球 阳性表达 人眼 

分 类 号：R394.2[医药卫生—医学遗传学]