Met-RANTES在rd小鼠遗传性视网膜变性中的作用  

The Effect of Met-RANTES in the Retial Dystrophy of Rd Mice

作　　者：陈星星[1] 曾惠阳[2] 刘谦[2] 卢清君[2] 唐胜建[1] 

机构地区：[1]潍坊医学院整形外科研究所,山东潍坊261042 [2]首都医科大学附属北京同仁医院北京同仁眼科中心

出　　处：《潍坊医学院学报》2014年第2期81-83,I0001,共4页Acta Academiae Medicinae Weifang

基　　金：国家自然科学基金项目(课题编号:81100675);北京市自然科学基金项目(课题编号:7102034)

摘　　要：目的研究CC趋化因子拮抗剂Met-RANTES对rd小鼠视网膜外核层形态的影响,探讨其在rd小鼠遗传性视网膜变性中的潜在治疗作用。方法以出生后7d rd小鼠作为研究对象,随机分为5组,分别给予腹腔注射Met-RANTES、PBS和玻璃体腔注射不同浓度Met-RANTES、PBS,直到rd小鼠出生后14d,过量麻醉法处死后摘取眼球制作冰冻切片,HE染色后观察测量视网膜后极部外核层厚度。结果腹腔注射met^RANTES、玻璃体腔注射0.7μl Met-RANTES rd小鼠与对照小鼠视网膜外核层细胞层数无明显变化(P>0.05)。玻璃体腔注射1.0μl Met-RANTES和1.5μl Met-RANTES眼球视网膜外核层比对照眼球视网膜外核层厚,有统计学差异(P<0.05)。结论 CC趋化因子拮抗剂Met-RANTES对rd小鼠遗传性视网膜变性可能存有潜在治疗作用。Objective To study the effect of chemokine antagonist Met-RANTES on outer nuclear layer reti-na in the retinal degenerative process in rd mice and further explore its role in the photoreceptor degeneration .Methods Rd mice at postnatal day （ P） 7 were enrolled and divided to 5 groups.The subjects were treated with intraperitoneal injection or intravitreous injection with Met-RANTES,PBS in different concentrations .The mice were sacrificed and reti-nal sections were prepared ,which were stained by hematoxylin-eosin staining （ HE） .Then the thickness of outer nuclear layer retinas were detected the thickness of outer nuclear layer retina respectively .The use and care of the animals com-plied with the guideline of ARVO .Results There was no evident diffiernce between the rd mice which were treated by Met-RANTES and their control group（P〉0.05）.So did the rd mice which were treated by 0.7μl Met-RANTES with glass body cavity injection .However,the rd mice which treated by 1.0μl or 1.5μl Met-RANTES were obviously thicker than their control retinas （ P〉0 .05 ） .Conclusion Chemokine antagonist Met-RANTES may poly a therapeutic role in the retinal degenerative process in rd mice .

关 键 词：视网膜变性 CC趋化因子拮抗剂 Met—RANTES rd 小鼠 自发性遗传性视网膜变性 

分 类 号：R774.1[医药卫生—眼科]