脑缺血再灌注后大鼠运动皮质SIRT_3的表达特点  

Expression of SIRT_3 in the cerebral motor cortex of rats following cerebral ischemic reperfusion

作　　者：常明则 吴海琴[2] 乔琳 王新来[1] 狄政莉[1] 刘志勤[1] 毛佳[1] 田晔[1] 

机构地区：[1]西安市中心医院神经内科,西安710003 [2]西安交通大学第二医院神经内科 [3]西安市第五医院麻醉科

出　　处：《陕西医学杂志》2014年第5期526-528,共3页Shaanxi Medical Journal

基　　金：国家自然科学基金面上项目(81270415);陕西省卫生厅科研基金(2012D43);西安市科技计划项目(SF1319(2))

摘　　要：目的:探讨全脑缺血再灌注大鼠大脑运动皮质内沉默信息调节因子3(SIRT3)的表达规律。方法:应用免疫组织化学染色方法测定全脑缺血再灌注后不同时间点(6h、24h、72h、5d及7d组)大鼠大脑运动皮质内SIRT3的表达情况。结果:6h组、24h组至72h组脑缺血再灌注大鼠大脑运动皮质内SIRT3的含量随再灌注时间延长而增加(P<0.01);72h组、5d组至7d组大鼠大脑运动皮质内SIRT3含量随再灌注时间延长而减少(P<0.01)。结论:全脑缺血再灌注后大鼠大脑运动皮质内SIRT3的表达随再灌注时间延长呈抛物线改变,提示SIRT3可能参与脑缺血再灌注损伤的病理生理过程。Objective：To observe the changes in the expression of silent information regulator 3 （SIRT3 ） in the cerebral motor cortex of rats following global cerebral ischemic reperfusion .Methods：The cerebral tissues were collected from rats following global cerebral ischemic reperfusion at time point of 6h ,24h ,72h ,5d ,and 7d （7 in each group） ,and the expression of SIRT3 in the cerebral cortex was detected by immunohistochemistry .Results ：Under optical microscope ,SIRT3-positive cells were found in every group .Within the time range of 6h ,24h and 72h following global cerebral ischemic reperfusion ,SIRT3-positive cells increased significantly （P〈0 .01） ,but within the time range of 72h ,5d and 7d ,the number of SIRT3-positive cells reduced significantly （P〈0 .01） .Conclusion ：The parabola-like change of SIRT3 expression time-phase in the cerebral cortex indicate that SIRT 3 might be related to pathophysiological processes of cerebral ischemic reperfusion .

关 键 词：缺氧缺血 脑 病理生理学 再灌注 信息素类 代谢 大鼠@沉默信息调节因子3 

分 类 号：R743.3[医药卫生—神经病学与精神病学]