桉树林地与甘蔗地微生物热代谢活性比较  

Comparison of Microbial Thermal Activity under Two Different Land Management(Eucalyptus granddis×E.uophyllaand Sugarcane)

作　　者：杨林林[1] 江绍锋 于晓宇[1] 夏樱花 蓝运华 陆祖军[1,2] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004 [2]珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2014年第1期141-148,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31060120);广西自然科学基金资助项目(2013GXNSFAA019059);广西教育厅科学技术研究项目(2009-25);珍稀濒危动植物生态与环境保护省部共建教育部重点实验室研究基金资助项目(桂科能1101K001);中国教育部和国家科技支撑计划(2012BAC16B03)

摘　　要：微量热法被用于分析连续种植桉树Eucalyptus granddis×E.uophylla、甘蔗的地块的土壤微生物代谢活性。于雨季和旱季分别从接壤的种植桉树、甘蔗地块采集土壤样品(样地大小10m×10m,各采500g),加入含5.0mg葡萄糖和5.0mg硫酸铵的0.6mL溶液的1.2g土壤样品被用于微量热法实验,温度设定为28℃;另外,土壤的理化和生物学特性也被测定,用于共同反映土地经营模式对土壤质量的影响。结果:(1)无论是雨季还是旱季,甘蔗连续种植地土壤内发酵型细菌为土壤微生物主要类群,但桉树林地只有雨季时发酵型细菌占优势;(2)与甘蔗连续种植地相比,桉树林地的土壤密实度较低,土壤微生物组成和代谢活动的季节性波动程度较高;(3)相对于桉树,甘蔗的连续种植对土壤微生物代谢活性产生较大的抑制作用。The microcalorimetric method was applied to analyze the influences of successive reforestations with Eucalyptus granddis X E. uophylla and continuous sugarcane production on the soil microbial activities. 500 g of each refined representative sample was collected in rainy and dry seasons from two 100 m2 homogeneously and perfectly defined acrisol quadrats in subtropical China. Mierocalorimetric experiments were carried out using 1.2 g soil samples and 0. 6 mL of solution containing 5. 0 mg of glucose and 5.0 mg of ammonium sulphate at 28 ℃ ;also the effects of land management practice on soil quality were examined by measuring their physicochemical and biological properties. The results showed that： （1） zymogeneous bacteria were the dominated microbes in the land of continuous sugarcane production,but autochthonous flora were the ones for Eucalyptus granddis X E. uophylla; （2） when compared with land of sugarcane, eucalyptus land had lower soil packing degree but higher seasonal fluctuation in microbe constitution and activities under the same circumstance; （3） against eucalyptus, sugarcane continuous production exerted higher degree of inhibitory on the soil microbial metabolism.

关 键 词：微量热法 土壤微生物 桉树 甘蔗 

分 类 号：X171.4[环境科学与工程—环境科学]