TGF-β1对人粘连成纤维细胞增殖与COL-I合成的影响  

Effect of TGF-β1 on the proliferation and COL-I synthesis of human fibroblasts from adhesive tissues

作　　者：王芳[1] 毛建文[2] 曾煦欣[3] 褚夫江[2] 时军[1] 王小燕[1] 崔升淼[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广东药学院基础学院,广东广州510006 [3]佛山科学技术学院医学院,广东佛山528000

出　　处：《广东药学院学报》2014年第1期103-105,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省卫生厅课题(B2012184);广东省教育厅育苗工程(LYM10132);佛山市科技发展专项资金(201002089)

摘　　要：目的考察TGF-β1对人粘连成纤维细胞（ATF）增殖与COL-Ⅰ合成的影响。方法通过人体腹膜粘连组织原代培养获取ATF，将不同剂量的TGF-β1与ATF共培养48h，MTT法测定细胞增殖活性，ELISA法测定培养基中COL-Ⅰ的含量。结果TGF-β1 0．5～40ng／mL剂量组的ATF增殖活性均高于0ng／mL剂量组，差异有统计学意义（P〈0．01）。TGF-β1 10、20ng／mL剂量组的COL-Ⅰ含量与0ng／mL剂量组差异有统计学意义（P〈0．01）。结论TGF-β1可明显提高人ATF的增殖活性与COL-Ⅰ合成水平。Abstract： Objective To study the effect of TGF-β1 on the proliferation and COL-I synthesis of human fibroblasts from adhesive tissue （ATF）. Methods ATF from human peritoneal adhesive tissues were harvested by primary culture. The proliferation of ATF treated with different dosage of TGF-β1 was measured by MTT assay. ELISA was employed to determine the content of COL-Ⅰ in ATF culture medium. Results Compared with the control group, ATF treated with the dosage from 0.5 to 40 ng/mL TGF-β1 showed an higher viability （P〈O.01）. Meantime, COL-I content was significantly increased when ATF treated with 10 and 20 ng/mL TGF-β1 （P〈0.01）. Conclusion TGF-β1 may increase the proliferation and COL-Ⅰ synthesis of human ATF.

关 键 词：TGF-Β1 成纤维细胞 粘连 

分 类 号：R363[医药卫生—病理学]