miR-125b对胶质瘤细胞侵袭能力影响的体内研究  

Research on miR-125b expression and invasion of glioma in vivo

作　　者：李晓良[1] 孙王伟[2] 张曙光[1] 万意[3] 石磊[1] 王之敏[3] 王存祖[4] 

机构地区：[1]江苏大学附属昆山医院神经外科,苏州215300 [2]江苏大学附属昆山医院检验科,苏州215300 [3]上海交通大学附属苏州九龙医院神经外科 [4]江苏省苏北人民医院神经外科

出　　处：《临床神经外科杂志》2014年第2期90-93,共4页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金项目资助(81000963);江苏省"333工程"培养资金资助项目(BRA2011046);江苏省卫生厅医学科研课题(YG201301);苏州社会发展项目基金(SYS201063);昆山社会发展项目基金(KS1006和KS1009);江苏大学医学临床科技发展基金项目(JLY20120053)

摘　　要：目的探讨体内miR-125b表达对人脑胶质瘤细胞侵袭力的影响及其可能的机制。方法培养原代胶质瘤细胞,分别转染miR-125b mimics和miR-125b inhibitor慢病毒载体,上调或下调细胞中miR-125b表达水平,种植乳鼠皮下,Transwell试验评价胶质瘤细胞侵袭能力的变化,Western Blot验证侵袭相关基质金属蛋白酶(MMP)-2、MMP-9及RECK和TIMP3蛋白的表达。结果转染miR-125b mimics能有效提高原代胶质瘤细胞中miR-125b的表达,miR-125b inhibitor有效降低miR-125b的表达;体内实验表明miR-125b mimics及inhibitor对胶质瘤侵袭能力无明显影响,不影响侵袭相关MMP-2、MMP-9及RECK、TIMP3蛋白的表达。结论体内miR-125b表达水平与原代胶质瘤细胞的侵袭能力无明显相关。Objective To investigate the function and possible mechanisms of miR-125 b expression on the invasion of glioma in vivo .Methods Primary glioblastoma cells were separated and cultured , which were transfected with lentiviral vectors for miR-21 mimics or miR-21 inhibitors for upregulation or downregulation of miR-125b expression levels.After that, cells were directly injected subcutaneously into the flanks of nude mice .The invasive effects of primary glioblastoma cells and stem cells were measured by Transwell assay , invasion related genes were screened by mRNA array, and the expressing levels of matrix metalloprotease （ MMP）-2, MMP-9, RECK and TIMP3 were identified by Western blot .Results miR-125 b mimics transfection in vitro could effectively improve the expression of miR-125 b in primary glioblastoma cells and stem cells , miR-125 b inhibitor transfection could effectively inhibit the expression of miR-125 b.Results showed that miR-125 b mimics or inhibitor had no effects on the invasion of primary glioblastoma cells in vivo , and did not affect the expression of MMP-2, MMP-9, RECK and TIMP3.Conclusion miR-125b expression is not related to the invasion properties of primary glioblastoma cells in vivo .

关 键 词：胶质瘤 miR-125b 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]