吖啶橙诱导酪氨酸酶构筑酚类碳毡传感器及电化学行为  

Construction of acridine orange induced tyrosinase-carbon felt-based phenol biosensor and its electrochemical behaviour research

作　　者：张志强[1] 雷蕾[1] 王涛[1] 李雨晴[1] 张楠[1] 陈佳慧[1] 宋云飞[1] 王月[1] 

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《辽宁科技大学学报》2014年第1期1-4,9,共5页Journal of University of Science and Technology Liaoning

基　　金：鞍山市民生科技进步行动计划项目(2011MS14)

摘　　要：酚类化合物是很多行业的生产废物,其残留对于人类的健康十分不利。因此,对环境中酚类化合物残留的快速准确地检测十分必要。研究使用酪氨酸酶为生物组分,通过吖啶橙诱导将其吸附在碳毡电极上,以环境污染物邻苯二酚及4-氯苯酚为底物,检测所制备电极的性能。研究表明,吖啶橙能够提高酪氨酸酶碳毡电极的性能,稳定及提高酪氨酸酶在碳毡电极表面的活性,对邻苯二酚及4-氯苯酚具有很好的检测性能。The phenol compounds are important contaminations generated from industrial waste products, which is causing problems for our living environment and showing adverse effects on human. It is necessary to build a rapid and accuracy detection for the environmental phenol compounds. In this research, the tyrosinase （TYR） adsorbed on the carbon felt （CF） electrode by co-adsorbed with acridine orange （AO）. The AO induced TYR-CF electrode is used to detect catechol and 4-chlorophenol. The results show that the presence of AO can enhance the characteristics of TYR-CF electrode, and increase the activity of TYR on the CF surface. It has a good detection function for catechol and 4-chlorophenol.

关 键 词：酪氨酸酶 吖啶橙 碳毡 传感器 酚类化合物 

分 类 号：O661[理学—分析化学]