温石棉及其代用纤维对鼠细胞钙激活蛋白43及B淋巴细胞瘤-2表达的影响  

Effect of chrysotile and its substitute on expressions of CAP43 and Bcl-2 in hamster cells

作　　者：王洪州[1,2] 邓丽娟[1,2] 王利民[1,2] 董发勤[3] 

机构地区：[1]四川绵阳四0四医院 [2]川北医学院附属第二医院呼吸内科,四川绵阳621000 [3]西南科技大学校长办公室,四川绵阳621000

出　　处：《实用医院临床杂志》2014年第3期49-52,共4页Practical Journal of Clinical Medicine

基　　金：国家自然科学基金资助项目(编号:49502025;40072020);四川省科技厅科研基金资助项目(编号:2010SZ0037)

摘　　要：目的研究温石棉及其代用纤维对鼠细胞钙激活蛋白43(calciumactinated protein 43,CAP43)及B淋巴细胞瘤-2(B-cell lymghoma,Bcl-2)表达的影响,探讨温石棉及其代用品的致癌性机制。方法将不同浓度的温石棉及其代用品[四川新康温石棉(A)、陕西陕南温石棉(B)、玻璃纤维(C)、陶瓷纤维(D)、硅灰石(E)、岩棉(F),阴性对照组(G)]暴露于V79细胞,使用免疫组织化学法检测CAP43及Bcl-2的表达,并使用SPSS 21.0对表达结果进行对比分析。结果 A、B组的仓鼠肺细胞中CAP43及Bcl-2表达增强较为显著,与其他组比较,差异有统计学意义(P<0.05);CAP 43及Bcl-2基因表达随着粉体浓度的增加,其OD值也呈上升趋势。结论温石棉及其代用纤维对于鼠细胞CAP43及Bcl-2表达有显著的增强作用。Objective To investigate the effect of chrysotile and its substitute on expressions of CAP43 and Bcl-2 in hamster cells and to explore the their possible carcinogenic mechanism. Methods Chinese hamster lung ceils（ V79 cells）were exposed to dif- ferent concentrations of chrysotile and its substitutes including chrysotile from Xinkang, Sichuan （ A）, chrysotile from Southern Shanxi （B）, glass fiber（ C ）, ceramic fiber（ D ）, wollastonite （E）and rockwool （F）. At the same time, cells without treatment were used as nega- tive controls. The expressions of CAP43 and Bel-2 in V79 cells were detected by using immunohistochemistry, and compared by using statistical program SPSS21.0. Results The expressions of CAP43 and Bcl-2 in A and B were significantly up-regulated （ P 〈 0.05 ）. As the concentration of powder increased,immune staining OD value of CAP43 and Bcl-2 were increased. Conclusion Chrysotile and its substitute can significantly influence the CAP43 and Bcl-2 expressions in hamster cells.

关 键 词：温石棉 代用品 肿瘤基因 钙激活蛋白43 B淋巴细胞瘤-2 

分 类 号：R733.4[医药卫生—肿瘤]