依达拉奉对脑出血大鼠血肿周围组织NF-κB p65蛋白表达的影响  

Effect of Edaravone on NF-κB p65 protein expression in perihematoma of rats with intracerebral hemorrhage

作　　者：赵彬[1] 王玉芳[2] 陈桂华[2] 张晋霞[2] 李世英[2] 刘玉玲[2] 张玉芹[2] 刘斌[2] 

机构地区：[1]河北联合大学校医院,河北唐山063000 [2]河北联合大学附属医院神经内一科,河北唐山063000

出　　处：《中国医药导报》2014年第15期17-19,共3页China Medical Herald

基　　金：河北省唐山市科学技术研究与发展计划课题(编号10150204A-24)

摘　　要：目的探讨依达拉奉对脑出血大鼠血肿周围组织NF-κB p65蛋白表达的影响。方法 108只健康雄性Wistar大鼠随机分为正常组、假手术组、脑出血组和依达拉奉治疗组,其中脑出血组和依达拉奉治疗组又分为3 h、6 h、12 h、1 d、2 d、3 d、5 d、7 d 8个亚组,每组6只。采用大鼠尾动脉自体不凝血50μL注入尾状核区建立脑出血模型。依达拉奉治疗组于模型制作成功后2 h开始腹腔内注射3 mg/kg依达拉奉,1次/d。免疫组化法检测NF-κB p65蛋白表达变化。结果与脑出血组相比,依达拉奉治疗组于出血后6 h、12 h、1 d、2 d、3 d、5 d、7 d血肿周围组织NF-κB p65蛋白表达均减少(P<0.05或P<0.01)。结论依达拉奉对脑出血后血肿周围脑组织损伤有保护作用,可能与其抑制NF-κB p65蛋白表达有关。Objective To investigate the effect of Edaravone on the NF-κB p65 protein expression in perihematoma of rats with intracerebral hemorrhage （ICH）. Methods 108 healthy adult Wistar rats were randomly divided into normal group, sham group, ICH group, Edaravon treatment group. The ICH group and Edaravon treatment group were randomly divided into 3 h, 6 h, 12 h, 1 d, 2 d, 3 d, 5 d, 7 d subgroups respectively after ICH, each group had 6 rats. ICH models were prepared by injecting 50 μL of autogenous blood into the right caudate nucleus of rats. Edaravon treatment group after ICH 2 h were given 3 mg/kg Edaravon by intraperitoneal injection, 1 time/d. The expression of NF-κB p65 protein was observed by immunohistochemistry. Results Compared with ICH group, the expression of NF-κB p65 protein in Edaravone treatment group were weakened at 6 h, 12 h, 1 d, 2 d, 3 d, 5 d and 7 d after ICH （P 〈 0.05 or P 〈 0.01）. Conclusion Edaravon has a protective effect on perihematoma brain tissues after ICH, it may be related to the inhibition of NF-κB p65 protein expression.

关 键 词：脑出血 NF-ΚB P65蛋白 依达拉奉 

分 类 号：R743.34[医药卫生—神经病学与精神病学]