肺腺癌组织微RNA表达谱及在CD133^+肺癌细胞中的表达研究  

Expression profile of microRNAs in lung adenocarcinoma tissue and its expression in CD133^+ lung cancer cells

作　　者：胡欣春[1] 徐建军[1] 喻东亮[1] 彭金华[1] 魏益平[1] 

机构地区：[1]南昌大学第二附属医院心胸外科,江西南昌330006

出　　处：《重庆医学》2014年第12期1409-1411,1415,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81160293);国家重大科学仪器设备开发专项(2011YQ170067);江西省教育厅青年科学基金资助项目(GJJ10067)

摘　　要：目的探讨肺癌组织中微RNA(miRNA)的表达谱以及在CD133+A549中的表达。方法取肺腺癌患者的肺癌组织标本和癌旁组织标本,进行miRNA表达芯片分析。利用流式细胞术(FCM)分选并培养CD133+A549细胞2周,检测CD133+、CD44+表达分析并应用实时荧光定量PCR检测miRNA-892b和miRNA-4686在CD133+A549细胞中的表达情况。结果肺癌组织中有27个miRNA表达较癌旁组织中的表达上调(P<0.05),6个miRNA的表达较癌旁组织中的表达下调(P<0.05)。CD133+A549细胞CD133+、CD44+双阳性表达率为75.0%。CD133+A549细胞中miRNA-892b和miRNA-4686的表达显著高于A549细胞(P<0.01)。结论肺腺癌组织中miRNA存在异常表达谱,miRNA-892b和miRNA-4686可能在维持CD133+肺癌细胞生物学特性中发挥着一定的作用。Objective To study the expression profile of microRNAs in the lung adenocarcinoma tissue and its expression in CD133＋ lung cancer cells .Methods The specimens of adenocarcinoma tissue and pericarcinomatous tissue were collected for con-ducting the microRNA expression chip analysis .The flow cytometry（FCM ） was adopted to sort and culture CD133＋ A549 cells in serum-free medium for 2 weeks .The positive rate of CD133＋CD44＋ in those cells was assayed .The expression of miRNA-892b and miRNA-4686 in CD133＋ A549 cells were detected by real-time qPCR .Results Compared with the pericarcinomatous tissue ,27 miRNAs expressions in the lung adenocarcinoma tissue were up-regulated（P＆lt;0 .05） and 6 miRNAs expressions were down-regula-ted（P＆lt;0 .05） .The positive rate of cancer stem like cells CD 133＋CD44＋ was 75 .0% .The expression of miRNA-892b and miRNA-4686 in CD133＋ A549 cells was higher than A549 cells（P＆lt;0 .01） .Conclusion Abnormal expression profile of miRNAs in lung ad-enocarcinoma tissue ,and miRNA-892b and miRNA-4686 may play a certain role to maintain the biological characteristics of CD133＋ lung cancer cells .

关 键 词：肺肿瘤 微RNAS CD133 

分 类 号：R734.2[医药卫生—肿瘤]