RNAi基因沉默对消化系统肿瘤化疗敏感性的影响  

Study on influence of RNAi gene silence on chemosensitivity of human digestive system neoplasms

作　　者：邵军[1] 余文静[1] 肖斌[1] 曾凡才[1] 

机构地区：[1]泸州医学院生物化学教研室,四川泸州646000

出　　处：《重庆医学》2014年第12期1471-1473,共3页Chongqing medicine

摘　　要：目的探讨RNAi基因沉默对消化系统肿瘤化疗敏感性的影响。方法选取胃癌SGC-7901细胞株和食管癌EC109细胞株,常规分为转染组和阴性对照组培养,转染组两株细胞株转染siRNA沉默Survivin的表达,并给予顺铂药物5mg/L共培养72h。空白对照组则给予常规培养,并给予转染组同剂量的顺铂共培养72h。MTT法检测两组细胞株体外增殖速度并比较其对化疗药物顺铂的敏感性。结果 siRNA沉默SGC-7901和EC109细胞株内Survivin的表达后,细胞对顺铂药物的敏感性提高,MTT结果提示细胞的增殖活性明显低于未沉默组细胞株,差异有统计学意义(P<0.05)。结论采用RNAi基因沉默技术可以干扰Survivin基因表达,抑制肿瘤细胞的增殖,同时显著提高肿瘤对化疗的敏感性。Objective To investigate the influence of RNAi gene silence on the chemosensitivity of human digestive system neo-plasms .Methods Esophageal cancer EC109 cells and gastric adenocarcinoma SGC-7901 cells were selected and divided into the transfection group and the blank control group for culturing .Two cell lines in the transfection group were transferred by siRNA and silenced the expression of Survivin ,giving cisplatin 5 mg/L to co-culture for 72 h .The blank control group was given the conven-tional culture and the same dose of cisplatin for co-culture for 72 h .The in vitro proliferation rate of cells in the two groups were measured by MTT and their sensitivities to the chemotherapy drug cisplatin were compared .Results After siRNA silencing the ex-pression of Survivin in SGC-7901 and EC109 cell lines ,the sensitivity of cells to cisplatin drugs was increased .MTT results sugges-ted that the proliferation activity of cells was significantly lower than that in the non-silence cell lines ,the difference showing statis-tical significance（P〈0 .05） .Conclusion Adopting RNAi gene silencing technique can interfere the expression of Survivin gene ,in-hibit the proliferation of tumor cell ,and at the same time significantly improve the sensitivity of tumors to chemotherapy .

关 键 词：RNA干扰 基因沉默 消化系统肿瘤 抗肿瘤联合化疗方案 敏感性 

分 类 号：R735[医药卫生—肿瘤]