miRNA-625*在膀胱尿路上皮癌中的表达  

Expression of miRNA-625* in urothelial carcinoma of the bladder

作　　者：徐锋[1] 韩从辉[2] 刘有黄[1] 高建平[1] 张征宇[1] 葛京平[1] 位志峰[1] 程文[1] 

机构地区：[1]南京军区南京总医院泌尿外科,江苏南京210002 [2]江苏省徐州市中心医院泌尿外科,江苏徐州221009

出　　处：《东南国防医药》2014年第2期121-123,共3页Military Medical Journal of Southeast China

基　　金：国家自然科学基金(30772278)

摘　　要：目的探讨miRNA-625*在膀胱尿路上皮癌中的差异表达。方法常规提取新鲜膀胱尿路上皮癌与正常膀胱黏膜组织中总RNA;使用分光光度计测定RNA在260 nm、280 nm和230 nm的吸光值,以计算浓度并评估纯度;用琼脂糖凝胶电泳检测RNA纯度及完整性;以RT-PCR方法检测标本中miRNA-625*的表达情况。结果 RNA质量检测合格后,miRNA-625*经RT-PCR验证与miRNA芯片分析结果一致。与正常膀胱黏膜上皮组织相比,膀胱尿路上皮癌中miRNA-625*表达显著上调(P<0.05)。结论 miRNA-625*在膀胱尿路上皮癌中表达上调,可作为膀胱尿路上皮癌诊断和预后判断的分子标志物。Objective To detect the miRNA-625＊expression in urothelial carcinoma of the bladder （ BUC） .Methods BUC and normal bladder mucosa tissues were collected and the total RNA was extracted routinely .Measuring the absorption value of RNA at 260 nm,280 nm,and 230 nm by NanoDrop ND-1000 in order to calculate RNA Quantification and Quality Assurance .RNA Integrity and gDNA contamination Test by Denaturing Agarose Gel Electrophoresis .The miRNA-625＊expression was detected by using real-time quantitative polymerase chain reaction .Results RNA Quantification and RNA Integrity were qualified for the follow-up experiment ,the miRNA-625＊ finding was confirmed by real-time RT-PCR and the expression was significantly up-regulated in BUC tissues than those in normal bladder mucosa （P＆lt;0.05）.Conclusion The expression level of miRNA-625＊is significantly up-regulated in BUC,and it may be used for prediction of prognosis and as for targeted treatment of BUC.

关 键 词：miRNA-625＊ 膀胱尿路上皮癌 微小RNA 

分 类 号：R737.14[医药卫生—肿瘤]