黄连素对SMMC-7721人肝癌细胞凋亡和细胞周期的影响研究  

The effects of berberine on human hepatoma cell SMMC-7721's cell apoptosis and cell cycle

作　　者：王丽芳[1] 陈垦[2] 臧林泉[2] 张海涛[2] 杜侃[2] 

机构地区：[1]广东药学院护理学院,广东广州510310 [2]广东药学院药科学院药理系,广东广州510006

出　　处：《临床和实验医学杂志》2014年第9期716-719,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的研究黄连素对SMMC-7721人肝癌细胞凋亡和细胞周期的影响,为临床肝癌的治疗提供科学的参照。方法选取人的SMMC-7721型肝癌细胞,分别在加入500μM、250μM、125μM、62.5μM、31.25μM、0μM黄连素的培养液中进行体外培养。于0 h、24 h、48 h、72 h时使用MTT方法对细胞活性进行测定,基于统计学处理方法,分析黄连素对SMMC-7721人肝癌细胞凋亡和细胞周期的影响作用。结果黄连素对SMMC-7721人肝癌细胞的生长具有显著的抑制作用,且随着作用时间和作用浓度的增加抑制作用呈上升趋势,但黄连素不会影响SMMC-7721人肝癌细胞的复制增殖周期。结论黄连素对能够显著抑制SMMC-7721人肝癌细胞的生长,并诱导细胞的凋亡,作用程度与作用时间和浓度呈正相关,但对细胞的增殖周期没有影响。Objective To study the effects of berberine on apoptosis and cell cycle of human hepatocellular carcinoma SMMC - 7721 cells,and provide scientific reference for clinical treatment of hepatocellular carcinoma（hcc）. Methods SMMC - 7721 hepatocarcinoma cell people,were cultured in medium with 500 μM,250 μM,125 μM,62. 5 μM,31. 25 μM,0 μM of berberine in. In 0 h,24 h,cell activity using the MTT method were measured by 48 h,72 h,statistical analysis based on the method. The effect of berberine on apoptosis and cell cycle of hu-man hepatocellular carcinoma cells SMMC - 7721 was analyzed. Results Berberine has obvious inhibitory effect on SMMC - 7721 human liver cancer cell growth. With the increase of concentration and action time of the inhibition,berberine showed an upward trend,but will not affect the replication cycle of human hepatocellular carcinoma SMMC - 7721 cells. Conclusion Berberine can inhibit the SMMC - 7721 on human hepato-cellular carcinoma cell growth,and induce cell apoptosis. The effect was positively related to the degree and affect time and concentration,but no effect on cell proliferation cycle.

关 键 词：SMMC - 7721 人肝癌细胞 黄连素 细胞凋亡 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]