洋葱假单胞菌G4对三氯乙烯的降解特性研究  

Characteristics of Trichloroethylene Degradation by Burkholderia cepacia. G4

作　　者：李利文[1,2] 甘义群[1,2] 周爱国[1] 韩莉[1,2] 于凯[1,2] 余婷婷[1,2] 

机构地区：[1]中国地质大学(武汉)环境学院 [2]中国地质大学(武汉)生物地质与环境地质教育部重点实验室,湖北武汉430074

出　　处：《环境科学与技术》2014年第5期1-4,9,共5页Environmental Science & Technology

基　　金：国家自然科学基金项目(41072179;40772156)

摘　　要：三氯乙烯(TCE)是地下水中最常检测到的有机污染物之一,且具有疑似致癌性,会对人类健康产生很大的危害。生物修复因其花费低,并能将TCE彻底降解而被广泛关注。Burkholderia cepacia.G4是一种能有效降解TCE的微生物菌株。文章从共代谢基质种类、接种时期、耐受性3个方面研究了G4菌株对TCE的降解特性。研究结果表明,对于3种共代谢基质—葡萄糖、苯酚和甲苯,G4菌株能够以甲苯和苯酚为基质进行TCE的代谢降解,而无法利用葡萄糖进行共代谢降解;苯酚-TCE共代谢降解速率高于甲苯-TCE的共代谢降解速率;G4以苯酚-TCE为共代谢基质,其在稳定期对TCE的降解速率要高于对数期,降解速率为1 040μg/(L·h);G4菌株对TCE的耐受浓度约为10 mg/L。Trichloroethylene （TCE） as one of the most frequently detected groundwater contaminants is harmful to human health. One of the most promising clean-up technologies is bioremediation because of its low cost and its potential for complete degradation of trichloroethylene. Burkholderia cepacia.G4 is capable of degrading trichloroethylene. The impact factors of Burkholderia cepacia.G4 degrading tdchloroethylene were studied including the co-metabolism materials, inoculation time and tolerance. Results showed that trichloroethylene was degraded with toluene and phenol as the cometabolize substrate while glucose could not. The degradation rate on stability phase was 1 040 μg/（L-h）, which was higher than the log phase when using phenol as the co-metabolism substrate. The tolerance of Burkholderia cepacia.G4 to TCE was about 10 mg/L.

关 键 词：BURKHOLDERIA cepacia-G4 三氯乙烯(TCE) 共代谢 降解 

分 类 号：X132[环境科学与工程—环境科学]