海滨锦葵提取物体外抗氧化作用研究  

In vitro antioxidant activities of extracts from Kosteletzkya virginica (L.) Presl

作　　者：吴双[1,2] 印敏 单宇 陈雨[1,2,3] 邱萍 赵友谊 王鸣 冯煦 

机构地区：[1]江苏省盐土生物资源研究重点实验室,江苏省中科院植物研究所,南京210014 [2]江苏省药用植物研究开发中心,江苏省中国科学院植物研究所,南京210014 [3]中国科学院南京分院东台滩涂研究院,东台224200

出　　处：《食品科技》2014年第5期193-197,共5页Food Science and Technology

基　　金：江苏省自然科学基金项目(BK2011424;BK20131338);江苏省药用植物研究开发中心开放基金项目(药201101);江苏省盐土生物资源研究重点实验室开放课题(JKLBS2012006)

摘　　要：目的:研究海滨锦葵不同器官不同溶剂提取物的体外抗氧化作用。方法:利用超声辅助提取法分别得到海滨锦葵根、茎、叶、花、种子的正己烷、二氯甲烷、乙酸乙酯和甲醇提取物,采用亚硝酸钠-硝酸铝[Na2NO2-Al(NO3)3]显色法检测各提取物中总黄酮含量,采用FolinCiocalteu法检测各提取物中总酚含量,通过1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)清除能力,对各提取物的体外抗氧化活性进行评价。结果:海滨锦葵不同器官不同溶剂提取物抗氧化活性有差异,其中根的乙酸乙酯提取物、茎的二氯甲烷提取物抗氧化活性相对较强,其总黄酮含量分别达到49.24、53.89 mg/g,总酚含量分别为50.39、43.46 mg/g。结论:海滨锦葵可作为天然的抗氧化物质资源,具有潜在的开发利用价值。Objective： The aim of this study was to investigate the antioxidant activities of different parts and different solvent extracts from Kosteletzkya virginica （L.） Presl. in vitro. Methods： Using ultrasound- assisted extraction method, the N-hexane, dichloromethane, ethyl acetate and methanol extracts from roots, stems, leaves, flowers and seeds of Kosteletzkya virginica （L.） Presl. were obtained, respectively. The total flavonoids content of various extracts were detected with Na2NO2-Al（NO3）3 chromogenic system.The total phenolics content of various extracts were detected with Folin-Ciocalteu assay. The antioxidant activity of various extracts in vitro were evaluated by 1,1-diphenyl-2-picrylhydrazyl free radical （DPPH.） system and hydroxyl radical （-OH） system. Results： The antioxidant activities of ethyl acetate extracts of roots and dichloromethane extracts of stems were stronger than other extracts; their total flavonoids content were 49.24, 53.89 mg/g, respectively; their total phenolics content to reach 50.39, 43.46 mg/g, respectively. Conclusion： Kosteletzkya virginica （L.） Presl. could be used as natural antioxidant resources with a good development and utilization value.

关 键 词：海滨锦葵 抗氧化 总黄酮 总酚 

分 类 号：R285[医药卫生—中药学]