维生素C对纳米二氧化钛致突变的保护作用研究  

The protective effect of vitamin C on the mutagenic toxicology of nano-titanium dioxide in mice

作　　者：王亚宁[1] 关秋艳 谢吕 郭会彩[1] 刘诣[1] 侯玉春[1] 张荣[1] 牛玉杰[1] 

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017

出　　处：《工业卫生与职业病》2014年第3期187-190,193,共5页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金(81072269;81102151);河北省自然科学基金(C2011206048);国家级大学生创新创业训练计划项目(201310089003)

摘　　要：目的观察维生素C对50nm TiO2致小鼠体细胞和生殖细胞突变的保护作用。方法将体质量为20-26g（精子畸形实验小鼠为15～18g）的健康小鼠150只随机分为5组，分别为阴性对照组（生理盐水）、50nm TiO2组（剂量为2g／kg）和高、中、低3个维生素C＋50nm TiO2组（维生素c剂量分别为100、200、400mg／kg，50nm TiO2剂量为2g／kg），连续灌胃染毒5d，1次／d，于末次染毒后24h观察小鼠骨髓细胞染色体畸变率、外周血微核率、骨髓细胞微核率、睾丸染色体畸变率，末次染毒后第36天观察精子畸形率。结果高、中维生素C＋50nm TiO2组外周血微核率明显低于50nm TiO2组（P〈0．05）。各不同剂量维生索C＋50nm TiO2组骨髓微核、骨髓染色体畸变、睾丸染色体畸变以及精子畸形率均明显低于50nm TiO2组，差异有统计学意义（P〈0．05）。结论维生素C对50nm TiO2致小鼠体细胞的和生殖细胞突变具有一定的保护作用。Objective To observe the protective effect of vitamin C on the mutagenic toxicology of 50 nm titanium dioxide in mice. Methods One hundred and fifty mice with body weight 20-26 g were randomly divided into five groups： the control group, 50 nm TiO2 group （2 g/kg）and three Vitamin C ＋50 nm TiOz groups （100 mg/kg,200 mg/kg,400 mg/kg,respectively）. The mice were treated by intragastric administra- tion. Chromosome aberration rate and micronucleus rate of bone marrow cells, micronucleus rate of erythrocyte, chromosome aberration rate in testicle and sperm abnormality rate were observed. Results The micronucleus rate of erythrocyte in the victamin C ＋50 nm TiO2 group at the concentration of 200 and 400 mg/kg was significantly decreased compared with the nano-titanium dioxide group （P〈0.05）. Chromosome aberration rate and micronucleus rate of bone marrow cells, chromosome aberration rate in testicle and sperm abnormality rate in the victamin C group at the different concentration were significantly decreased compared with the nano-titanium dioxide group （P〈0.05）. Conclusions Vitamin C antagonises the genetic toxicity induced by nano-titanium dioxide in somatic cells and germ cells of mice.

关 键 词：纳米TIO2 维生素C 微核试验 染色体畸变 精子畸形 

分 类 号：R135[医药卫生—劳动卫生]