颅内静脉窦血栓形成动物模型建立及治疗实验研究  

CVST Animal Models Establishment and Experimental Treatment Research

作　　者：汤恒心 曹志恺[2] 吕建平[2] 

机构地区：[1]广州市南沙中心医院,广东广州511457 [2]广州市第一人民医院

出　　处：《中国医学创新》2014年第12期16-19,共4页Medical Innovation of China

基　　金：广州市医药卫生科技项目(20131A011031);福建省科技计划重点项目(2010Y0043)

摘　　要：目的:通过建立上矢状窦(SSS)血栓形成的动物模型,并进行溶栓、抗凝治疗,观察其治疗效果。方法:清洁级青紫蓝大白兔分三组:A组为对照组;B、C两组分别为经颈动脉、SSS溶栓治疗组。第一步用可吸收缝线结扎及用血管夹夹闭SSS制作颅内静脉窦血栓形成(CVST)动物模型,经两种途径溶栓治疗CVST,检测目标静脉窦血流速度、窦旁脑组织含水量、血流变,随后进行抗凝维持治疗。1个月后行DSA及经颅多普勒(TCD)检查。结果:新动物模型可重复、稳定建立。C组脑组织含水量、基底静脉血流速度与A、B两组比较差异均有统计学意义(P<0.05);B、C两组血流变均有变化,但差异不明显;C组远期静脉窦血流再通比例优于B组。结论:该动物模型模拟SSS血栓形成,可满足CVST实验研究的需要;直接静脉窦途径溶栓治疗CVST近、远期效果优于经颈动脉途径溶栓。Objective：To study the effects of injection of urokinase and anticoagulation in treatment of CVST with new animal models. Method：Clean level chinchilla white rabbits were divided into three groups：the group A as the control group;the group B and C were respectively by the carotid artery,SSS thrombolysis treatment group. First step with absorbable suture ligation and with vascular clamp GaBi SSS made intracranial venous sinus thrombosis（CVST）animal model,the two ways of CVST thrombolysis treatment,detection target sinus blood flow velocity,sinus brain tissue water content,hemorrheology and subsequent anticoagulant therapy to maintain. 1 month after DSA and transcranial doppler （TCD）examination. Result：The new animal model was performed with good reproducibility and stability. The brain water content,basal veins blood flow velocity of the group C were significantly lower than those of the group B and the control group A（P＆lt;0.05）;the hemorrheology in the group B and C were changed,but the differences were not obvious. After venous sinus blood flow recanalization rate of the Group C was better than that the group B. Conclusion：The new animal model resemble clinical CVST,thus being approriate for the the experiment research of CVST. Thrombelysis via superior sagittal sinus is better than thrombelysis via carotid artery.

关 键 词：颅内静脉窦血栓形成 动物模型 介入溶栓 脑组织含水量 

分 类 号：R-332[医药卫生]