芦丁对大鼠缺血再灌注心肌组织iNOS和eNOSmRNA表达的影响  

Effect of Rutin on the Expression of eNOS and iNOS mRNA in Myocardial Tissues of Rats with Ischemia Reperfusion Injury

作　　者：钱国强[1] 丁晶晶[1] 尹晓峰[1] 李茹[1] 赵国平[2] 

机构地区：[1]黄淮学院护理学系,河南驻马店463000 [2]暨南大学医学院,广州510632

出　　处：《中西医结合研究》2014年第2期79-81,共3页Research of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金(No.81173189);河南省科技攻关项目(No.122102310473);黄淮学院青年骨干教师资助计划

摘　　要：目的研究芦丁对大鼠心肌缺血再灌注损伤的保护作用及其机制。方法通过左冠状动脉结扎建立SD大鼠心肌缺血再灌注模型,将大鼠随机分为对照组、模型组、芦丁组、L-NAME(eNOS抑制剂)组,ELISA法检测血清一氧化氮(NO)水平,免疫抑制法检测血清肌酸激酶同工酶(CK-MB)浓度,实时荧光定量PCR分析各组心肌组织iNOS和eNOS mRNA表达的变化。结果与模型组比较,芦丁组血清NO水平增高(P<0.01),CK-MB浓度下降(P<0.01),eNOS mRNA表达升高(P<0.01),iNOS mRNA表达下降(P<0.01)。结论芦丁缺血预处理对心肌起到了保护作用,其机制可能与上调心肌组织eNOS mRNA、下调iNOS mRNA表达有关。Objective To investigate the protective effect of Rutin on myocardial tissue of rats with ischemia-reperfusion injury.Methods Rat model of ischemia-reperfusion injury was set up by ligating the left anterior de-scending batch of the coronary artery.SD rats were randomly divided into control group ,model group ,Rutin group and L-NAME （eNOS inhibitor） group.Serum level of NO was determined by ELISA and CK-MB concentration was examined by immun osuppression assay.The expression of iNOS and eNOS mRNA in myocardial tissues was evaluated by real-time quantitative PCR analysis.Results Compared with model group ,serum level of NO was in-creased while CK-MB concentration was significantly decreased in rats of Rutin group （P 〈0.01）.The expression of eNOS mRNA was up-regulated while the expression of iNOS mRNA was down-regulated in myocardial tissues of rats in Rutin group （P〈0.01）.Conclusion Ischemic preconditioning with Rutin is effective in the treatment of ischemia-reperfusion injury.The mechanism might be associated with regulating the expressions of eNOS and iNOS mRNA in myocardial tissues.

关 键 词：芦丁 一氧化氮 诱导型一氧化氮合酶 内皮型一氧化氮合酶 肌酸激酶同工酶 

分 类 号：R285.5[医药卫生—中药学]