NF-κB介导热休克蛋白70减轻大鼠急性坏死性胰腺炎的实验研究  

Hsp70Relieves Acute Necrotizing Pancreatitis via NF-κB Mediation in Rats

作　　者：程书榜[1] 李林林[1] 朱量[1] 秦颖[1] 

机构地区：[1]深圳市第二人民医院胃肠外科,广东深圳518035

出　　处：《南昌大学学报（医学版）》2014年第2期17-19,F0003,共4页Journal of Nanchang University：Medical Sciences

基　　金：广东省科技局资助项目(2011B031800355)

摘　　要：目的探讨热休克蛋白70(heat shock protein 70,Hsp70)减轻大鼠急性坏死性胰腺炎的机制。方法将60只SD大鼠按随机数字表法分为3组,每组20只。A组急性坏死性胰腺炎(aute nicrosis apoptonisis,ANP)模型由5%牛黄胆酸钠大鼠胰胆管逆行注射诱发而成,B组手术进腹后翻动十二指肠,C组使用温生理盐水持续腹腔灌洗30min,10h后同A组造ANP模型。于最后1次手术后6h处死大鼠,检查各组大鼠血清细胞因子IL-6、TNF-α和IL-8及血清淀粉酶;取胰腺组织行病理检查,并分别使用PCR和免疫组织化学方法检测胰腺中Hsp70和NF-κB的表达。结果 C组大鼠血清淀粉酶为(2 845.70±204.78)U·L-1,明显低于A组的(4 064.93±188.97)U·L-1,高于B组的(1 221.47±54.58)U·L-1(均P<0.05);C组大鼠血清TNF-α、IL-6、IL-8明显低于A组,高于B组(均P<0.05);C组大鼠胰腺组织Hsp70mRNA RQ值明显低于A组,但高于B组(P<0.05);NF-κB在胰腺中的表达:A组部分细胞核强阳性染色,胞浆中弱阳性染色,B、C组细胞核染色明显减弱,胞浆则未见染色。结论 HSP70可以通过调控NF-κB从而下调细胞因子的表达,进而改善大鼠ANP病理生理。Objective To explore the mechanism of action of heat shock protein 70（Hsp70）in relieving acute necrotizing pancreatitis（ANP）.Methods Sixty SD rats were randomly divided into three groups,with 20 rats in each group.In group A,ANP was induced by retrograde injection of 5% sodium taurocholate into pancreatic duct.In group B,the duodenum was flipped after abdominal surgery for the sham operation.In group C,ANP was induced 10 hours after continuous peritoneal lavage with warm saline for 30 minutes.The rats were sacrificed 6 hours after the last operation.Serum levels of tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,interleukin-8（IL-8）and amylase were measured by ELISA.In addition,pancreas tissues were obtained to examine the pathological changes.HSP70 expression was examined by PCR and nuclear factor-κB（NF-κB） expression by immunohistochemistry.Results The levels of serum amylase in group C[（2 845.70± 204.78）U·L^-1 ）]were lower than those in group A[（4 064.93±188.97）U·L^-1 ],but higher than those in group B[（1 221.47±54.58 ）U·L^-1 ]（all P ＆lt;0.05）;The levels of serum TNF-α, IL-6 and IL-8 in group C were lower than those in group A,but higher than those in group B（all P ＆lt;0.05）;The RQ of Hsp70 mRNA in group C was lower than that in group A,but higher than that in group B（P ＆lt; 0.05）;Immunohistochemistry showed strong positive nuclear staining and weak positive cytoplasmic staining for NF-κB in group A,and weak positive nuclear staining and＆amp;nbsp;negative cytoplasmic staining for NF-κB in group B and C.Conclusion HSP70 can decrease cytokine expression and reduce pathophysiological damage through down-regulating the expression of NF-κB in ANP rats.

关 键 词：NF-ΚB 热休克蛋白70 急性坏死性胰腺炎 机制 实验 动物 大鼠 

分 类 号：R-332[医药卫生] R576