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出 处:《重庆医学》2014年第13期1594-1596,共3页Chongqing medicine
基 金:重庆市医学科研计划基金资助项目(2010-2-474)
摘 要:目的观察转染抑癌基因KAI1后对人肾透明细胞癌786-O细胞的增殖及凋亡等生物学效应的影响。方法通过慢病毒载体将KAI1基因转染入786-O细胞中,使用荧光显微镜观察KAI1基因转入情况;应用噻唑蓝(MTT)方法检测慢病毒载体携带的KAI1基因转染786-O细胞后的体外增殖情况;流式细胞技术(FCM)检测KAI1诱导的细胞凋亡情况。结果当慢病毒载体转染的量为MOI-10,在荧光显微镜下可观察到转染后细胞有大量荧光表达,MTT及FCM检测KAI1对786-O细胞有显著的抑制作用,并可诱发786-O细胞凋亡的发生。结论 KAI1通过启动786-O细胞发生凋亡而抑制了肿瘤细胞的增殖。Objective To investigate the effects of KAI1 on renal cell carcinoma 786-O cells and apoptosis,study on the effect of HAI1 on 786-O growth inhibition.Methods The 786-O cells were treated with viral vector carried HKAI1 gene.The MTT test was used to evaluate the proliferation inhibition of HAI1 on 786-O cells.The flow cytometry were utilized to observe and detect the apoptosis of 786-O cells induced by KAI1.Results When the amount of lentiviral vector-transfected for MOI-10,could be observed under a fluorescence microscope after transfection tumor cell fluorescence expression,MTT and FCM detection shows that the KAI1 could significantly inhibit the proliferation and induce apoptosis of 786-O cells.Conclusion The proliferation inhibiting effects of KAI1 on human renal cell carcinoma cell line 786-O may relat to the apoptosis.
关 键 词:kangai-1蛋白 786—O 细胞增殖 细胞凋亡
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