非水毛细管电泳检测辣椒粉中苏丹红  

DETECTION OF SUDAN DYES IN CHILI POWDERS BY NONAQUEOUS CAPILLARY ELECTROPHORESIS

作　　者：黄露[1] 

机构地区：[1]闽江学院化学与化学工程系,福建福州350108

出　　处：《井冈山大学学报（自然科学版）》2014年第2期23-26,共4页Journal of Jinggangshan University (Natural Science)

基　　金：福建省自然科学基金项目(2010J05029);福建省教育厅科技计划项目(JB11142)

摘　　要：首次采用非水毛细管电泳法对两种苏丹红染色剂-苏丹红Ⅰ与苏丹红Ⅱ进行了分离检测。考察了电泳介质、背景电解质、SDS浓度对分离的影响。当运行缓冲液为100 mmol/L乙酸钠＋35 mmol/L SDS的乙腈/甲醇（6：4， v/v）混合溶液时，两种苏丹红化合物能够在15 min内达到基线分离。各组分的峰面积与待测物浓度在0.01-0.5 mg/mL范围内呈良好的线性关系，检测限均为0.005 mg/mL。最终检测到辣椒样品中含有苏丹红Ⅱ浓度为3.040 mg/g，苏丹红Ⅰ与苏丹红Ⅱ在加标辣椒样品中的回收率分别为94.68%和81.00%。Nonaqueous capillary electrophoresis was applied in the separation and determination of Sudan dyes （Sudan Ⅰ and Sudan Ⅱ）. The effects of the electrophoretic medium, background electrolyte and SDS concentration on the separation were investigated. When the running buffer was acetonitrile/methanol （6：4, v/v） containing 100 mmol/L sodium acetate and 35 mmol/L SDS, two Sudan red compounds could be basically separated and detected in 15 min. Excellent linearity was observed in the range from 0.01-0.5 mg/mL for these two analytes, and the detection limits were both 0.005 mg/mL. Finally, 3.040 mg/g of Sudan Ⅱ was found in chili powders, and the recoveries of Sudan Ⅰ and Sudan Ⅱ in spiked chili powders were 94.68%and 81.00%, respectively.

关 键 词：非水毛细管电泳 辣椒粉 苏丹红I 苏丹红I 

分 类 号：O657.8[理学—分析化学]