FOX03a在三氧化二砷影响大肠癌细胞β—catenin表达中的作用  

Effects of FOXO3a on the expression of β- catenin in colorectal cancer cells exposed to As2O3

作　　者：韩燕燕[1] 张琳[2] 程慧敏[2] 

机构地区：[1]徐州医学院研究生学院2011级,江苏徐州221004 [2]徐州医学院病理学教研室

出　　处：《徐州医学院学报》2014年第4期258-261,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省高校自然科学基金（12KJD320006,07KJD310217）;徐州市科技局项目（XF11C1002011）

摘　　要：目的探究三氧化二砷（As2O3）对侵袭相关因子β—catenin表达的影响是否与FOXO3a有关。方法采用脂质体转染siRNA以及LY294002药物改变大肠癌SW620细胞中FOXO3a的表达，Western blot检测FOXO3a、β—catenin蛋白表达水平，RT—PCR检测FOXO3a、β—catenin mRNA表达水平。结果As2O3增加SW620细胞中FOXO3a蛋白及mRNA的表达，同时降低β—catenin蛋白及mRNA表达（P〈0．05）。FOXO3a基因干扰后，β—catenin蛋白及mRNA的表达上调（P〈0．05）。LY294002药物处理后，β—catenin表达下调（P〈0．05）。结论As2O3对人大肠癌SW620细胞中β—catenin表达的抑制作用可能与其增强FOXO3a的活性有关。Objective To investigate whether FOXO3a is involved in the changed expression of β - catenin in colorectal cancer SW620 ceils after exposure to arsenic trioxide （ As2O3 ）. Methods The expression of FOXO3a was changed by FOXO3a specific siRNA and LY294002. Then, the protein and mRNA expressions of FOXO3a and β- catenin in eoloreetal cancer SW620 cells were detected by western blot and RT -PCR, respectively. Results As2O3 can stimulate the protein and mRNA expressions of FOXO3a in SW620 cells, while reducing the expression of β - catenin at protein and mRNA levels （P 〈 0.05 ）. After FOXO3a gene silencing, the protein and mRNA expressions of β -catenin were obviously enhanced （ P 〈 0.05 ）. But LY294002 treatment could remarkably inhibit the expression of β - catenin at protein and mRNA levels. Conclusion The inhibitory effects of As2O3 on β - eatenin in eolorectal cancer SW620 cells may be associate with its ability to enhance the activity of FOXO3a.

关 键 词：三氧化二砷 大肠癌 FOXO3A β—catenin 

分 类 号：R735.34[医药卫生—肿瘤]