青藏高原土壤中链霉菌的分离鉴定及其抗菌活性研究  

Streptomyces strains in the soils of the Tibetan Plateau: Isolation,identification and antimicrobial activity

作　　者：张玲[1] 李师翁[1] 陈熙明[2] 刘光琇[2] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070 [2]中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室,甘肃兰州730000

出　　处：《冰川冻土》2014年第2期430-441,共12页Journal of Glaciology and Geocryology

基　　金：国家国际科技合作专项(2011DFA32520)资助

摘　　要：对从青藏高原采集的土壤样品中的链霉菌进行了分离鉴定,并对其抗菌活性进行了研究.结果表明:11份土样中分离出菌落形态特征明显不同的链霉菌相似菌株151株,对其中43株进行了16S rDNA测序分析,41株菌株与已知链霉菌的相似性在99%以上,确定为链霉菌属(Streptomyces)菌种,而菌株WDL-DM-5和TTH-DM-1与已知链霉菌的序列相似性分别为90.76%和95%.抗菌试验表明,68株链霉菌对革兰氏阳性菌和阴性菌有抗性,其中41株菌对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌都有明显拮抗性,表明青藏高原土壤中存在强拮抗性的链霉菌.Streptomyces strains were isolated from the soils of the Tibetan Plateau and identified. Their antimicrobial activity was investigated in the present study. 151 Streptornyces strains were isolated from the soils sampled from 11 sites in the Tibetan Plateau. Among them 43 strains had been identified using 16S rDNA sequencing method, of which 41 strains were found belonging to Streptomyces. The similarity search indicate that besides strains WDL-DM-5 and TTH-DM-1 have 90.76% and 95% similarity compared with a known Streptomyces strain, respectively, the strains have more than 99% similarity as compared with a known Streptomyces strain. The antimicrobial tests showed that 68 strains of Streptomyces have antimicrobial activity against Gram-positive bacteria and Gram-negative bacteria. Among them 41 strains are able to resist all the three kinds of indicator bac- teria Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, indicating that Streptomyces with strong antimicrobial activity exist in the soils from the Tibetan Plateau.

关 键 词：青藏高原 链霉菌 分离鉴定 抗菌活性 

分 类 号：Q938[生物学—微生物学]