HMME-PDT对牙骨质片及种植体表面Pg菌斑生物膜的影响  

Impact of HMME-PDT on Pg of the surface of implants and cementum tablets

作　　者：黄青[1] 张健[1] 王永兰[2] 阴慧娟[3] 

机构地区：[1]天津市口腔医院牙周科,300041 [2]天津医科大学口腔医院牙周科,300070 [3]中国医学科学院北京协和医学院生物医学工程研究所,300192

出　　处：《国际生物医学工程杂志》2014年第2期98-102,共5页International Journal of Biomedical Engineering

摘　　要：目的 探讨光动力疗法（PDT）对牙骨质片及种植体表面牙龈卟啉单胞菌（Pg）菌斑生物膜的杀灭效果,获得有效杀灭Pg的光敏剂和激光照射的合理剂量.方法 选用Pg标准菌株分别联合经全唾液处理后的无菌牙骨质片和无菌种植体共同培养,建立简易的体外人工牙骨质片和种植体表面细菌附着模型.以血卟啉单甲醚（HMME）为光敏剂,波长630 nm半导体激光为光源,照射牙骨质片表面细菌附着模型60 s后,计算菌落形成单位（CFU）,筛选出最佳激光能量密度（EDL）和HMME剂量,以筛选的合理剂量照射种植体表面细菌附着模型,并用扫描电镜观察PDT对种植体表面菌斑生物膜的影响.结果 当EDL为12J/cm2、HMME质量浓度为25 μg/ml时,PDT可有效杀灭Pg菌斑生物膜（（13.00±5.00） CFU）,且扫描电镜观察到种植体表面无损伤.结论 630nm半导体激光联合HMME介导的光动力疗法（HMME-PDT）对Pg菌斑生物膜有良好的杀灭效果,本实验使用的EDL与HMME剂量较小,有望作为临床治疗剂量.Objective To evaluate the bactericidal effect of diode laser on Porphyromonas gingivalis （Pg）,and to explore an optimized protocol for a safe dose of photodynamic therapy （PDT） to eliminate periodontal pathogens as well as the impact on the implant surfaces,so as to provide theoretical and experimental basis for PDT in periimplantitis therapy.Methods Artificial in vitro models were formatted by culturing Pg standard strain and ITI （International Team for Implantology） implants together in CDC broth.Then artificial in vitro models were treated by different doses of hematoporphyrin monomethyl ether （HMME） and different energy density of laser （EDL） for 60 s.The cultures were counted by colony form unit （CFU）,and SPSS 17.0 statistical software was used for data statistical analysis to select the best EDL and HMME dose.Finally,ITI implants were observed by scanning electron microscope （SEM） to evaluate the impact of HMME-PDT on Pg of implant surfaces.Results When EDL was 12 J/cm2 and mass concentration of HMME was 25 μg/ml,SEM observations showed that PDT could effectively kill Pg （（13.00±5.00） CFU）without damaging the implant surfaces.Conclusions PDT therapy combining 630 nm diode laser with photosensitizer HMME have good bactericidal effect on Pg,and the EDL and HMME dose is as small as the clinical applicable safe dose.

关 键 词：光动力疗法 半导体激光 血卟啉单甲醚 牙龈卟啉单胞菌 种植体周围炎 

分 类 号：R783.6[医药卫生—口腔医学]