天津地区慢性乙型肝炎患者病毒基因型的分析及临床意义  

Analysis and clinical significance of genotypes of patients with chronic hepatitis B virus in Tianjin

作　　者：刘纯[1] 刘伟[1] 王凯[1] 李代红[1] 

机构地区：[1]天津市第一中心医院输血科,天津300192

出　　处：《中华医院感染学杂志》2014年第10期2399-2401,共3页Chinese Journal of Nosocomiology

基　　金：天津市卫生局科技基金资助项目(2013KZ035)

摘　　要：目的调查医院慢性乙型肝炎患者外周血中乙型肝炎病毒基因型的分布,为临床治疗提供参考依据。方法采用基因测序方法对医院548例慢性乙型肝炎患者进行乙型肝炎基因型检测,同时检测乙型肝炎DNA、乙型肝炎血清学标志物及肝功能;应用SPSS19.0软件进行统计分析,计量资料比较采用t检验,计数资料比较采用χ2检验。结果 548例慢性乙型肝炎患者中C型433例占79.0%,B型109例占19.9%,D型有6例占1.1%;B型乙型肝炎患者的HBV-DNA载量为(2.5±6.8)×104 copies/ml,其中75例肝功能异常;C型乙型肝炎患者的HBV-DNA载量为(1.3±5.9)×105 copies/ml,其中311例肝功能异常。结论天津地区慢性乙型肝炎患者基因型以C型为主,其次为B型,D型极少,未检出其他型;B型和C型患者相比,肝功能异常者无明显区别,但C型患者的HBV-DNA载量明显高于B型。OBJECTIVE To investigate the distribution of genotypes in the peripheral blood of patients with chronic hepatitis B virus （HBV） in the hospital, so as to provide references for clinical treatment. METHODS Genotypes of the 548 patients with chronic HBV were detected by the gene sequencing method, meanwhile HBV-DNA, HBV serologic markers as well as the liver function were detected; SPSS19.0 software was adopted for statistical analy- sis, while T-test was adopted for comparative analysis of the measurement data and Chi-square test was adopted for comparative analysis of the enumeration data. RESULTS Of the total 548 patients with chronic HBV, patients with genotype C, B and D accounted for 79.0 % （n = 433）, 19.9 % （n = 109）, and 1.1% （n = 1 ）, respectively; the HBV-DNA load for patients with genotype B HBV was （2.5 ±6.8）×104 copies/ml, including 75 patients with the abnormal liver function; the HBV-DNA load for patients with genotype C HBV was （1.3±6.8）× 105 copies/ml, including 311 patients with the abnormal liver function. CONCLUSION Genotype C is the dominant in the patients with chronic HBV in Tianjin, followed by genotype B and few genotype D patients, and other geno- types were not detected; there is no significant difference in the patients with abnormal liver function by comparing genotype B with genotype C, but the HBV-DNA load of the genotype C patients is significantly higher than that of the genotype B patients.

关 键 词：乙型肝炎 基因型 血清学标志物 基因测序 

分 类 号：R512.62[医药卫生—内科学]