Preparation and quality assessment of high-purity ginseng total saponins by ion exchange resin combined with macroporous adsorption resin separation  

作　　者：ZHAO Yu-Nan WANG Zhong-Li DAI Jian-Guo CHEN Lin HUANG Yu-Fang 

机构地区：[1]Key Laboratory of Brain Research, Basic Medical College, Nanjing University of Traditional Chinese Medicine

出　　处：《Chinese Journal of Natural Medicines》2014年第5期382-392,共11页中国天然药物（英文版）

基　　金：supported by the Jiangsu Provincial Natural Science Foundation of China(No.BK2011815);Specialized Research Fund for the Doctoral Program of Higher Education(No.20103237120011);the"Qing Lan"Project from Jiangsu Provincial Framework Teacher Support Scheme

摘　　要：AIM： To prepare high-purity ginseng total saponins from a water decoction of Chinese ginseng root.METHOD： Total saponins were efficiently purified by dynamic anion-cation exchange following the removal of hydrophilic impurities by macroporous resin D101. For quality control, ultrahigh-performance liquid chromatography with a charged aerosol detector （CAD） was applied to quantify marker components. The total saponin content was estimated by a colorimetric method using a vanillin-vitriol system and CAD response. RESULTS： D201, which consisted of a cross-linked polystyrene matrix and -]N＋（CI-13）3 functional groups, was the best of the four anion exchange resins tested. However, no significant difference in cation exchange ability was observed between D001 （strong acid） and D 113 （weak acid）, although they have different functional groups and matrices. After purification in combination with D101, D201, and D 113, the estimated contents of total saponins were 107% and 90% according to the colorimetric method and CAD response, respectively. The total amount of representative ginsenosides Re, Rd, Rgl, and compound K was approximately 22% based on ultrahigh-performance liquid chromatography-CAD quantitative analysis. CONCLUSION： These findings suggest that an ion exchange resin, combined with macroporous adsorption resin separation, is a promising and feasible purification procedure for neutral natural polar components.AIM: To prepare high-purity ginseng total saponins from a water decoction of Chinese ginseng root. METHOD: Total saponins were efficiently purified by dynamic anion–cation exchange following the removal of hydrophilic impurities by macroporous resin D101. For quality control, ultrahigh-performance liquid chromatography with a charged aerosol detector(CAD) was applied to quantify marker components. The total saponin content was estimated by a colorimetric method using a vanillin–vitriol system and CAD response. RESULTS: D201, which consisted of a cross-linked polystyrene matrix and –N+(CH3)3 functional groups, was the best of the four anion exchange resins tested. However, no significant difference in cation exchange ability was observed between D001(strong acid) and D113(weak acid), although they have different functional groups and matrices. After purification in combination with D101, D201, and D113, the estimated contents of total saponins were 107% and 90% according to the colorimetric method and CAD response, respectively. The total amount of representative ginsenosides Re, Rd, Rg1, and compound K was approximately 22% based on ultrahigh-performance liquid chromatography-CAD quantitative analysis. CONCLUSION: These findings suggest that an ion exchange resin, combined with macroporous adsorption resin separation, is a promising and feasible purification procedure for neutral natural polar components.

关 键 词：Panax ginseng GINSENOSIDES Ion exchange resin Macroporous resin Quality assessment 

分 类 号：R284[医药卫生—中药学]