Purification of Bromelain Enzyme from Curaua （Ananaserectifofius LB Smith） White Variety, by Aqueous Two-Phase System PEG 4000/Potassium Phosphate  

作　　者：Juliana Ferrari Ferreira Dalva Sbruzzi Kleber Vgnio Gomes Barros Daniela Diniz Ehrhardt Elias BasileTambourg 

机构地区：[1]Department of Engineering of Chemical Systems, School of Chemical Engineering, State University of Campinas, Campinas 500,Brazil

出　　处：《Journal of Chemistry and Chemical Engineering》2014年第4期395-399,共5页化学与化工（英文版）

摘　　要：The Curaua. （Ananaserectifolius LB Smith） is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapa, Acre, Mato Grosso and Goia s, has two varieties, purple and white, and its fibers are used in automotive and textile industries due to its strength, softness and lightweight. Currently, only the fiber is used in industry, the rest is considered waste However, this residue contains compounds with important properties to be discovered and studied. An enzyme complex found in this residue is bromelain, a group of proteolysis＇ enzymes with application in several areas such as in the food, pharmaceutical and cosmetics industries. Today, its use is aiming the pharmaceutical industry, the production of ointments, gels, creams and lotions because they offer a wide range of therapeutic efficacies： antiedemas, anti-inflammatory, antithrombotic and fibrinolytic activities. This work studied the enzyme purification and recovery presented in the leaves of Curaua by means of an ATPS （aqueous two-phase system） PEG 4000/potassium phosphate. The protein content was measured by Bradford reagent and the enzymatic activity was measured by the Biuret reagent. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. It was used pH 7.0, 8.0 and 9.0, varying the proportional composition between the polymeric and the saline phases （tie-lines）. The white variety of Curaua. was used. The best purification factor was 4.53 and about 113.04 μg/mL of total protein measured by Bradford.

关 键 词：Curaua PURIFICATION bromelain. 

分 类 号：TQ126.35[化学工程—无机化工] TS201.25[轻工技术与工程—食品科学]