沙地柏多酚的纯化及其抗氧化活性测定  

Purification of polyphenols from sabina vulgaris antoine and its antioxidant properties

作　　者：李龙[1,2] 宣贵达[1] 陈平[3] 

机构地区：[1]浙江大学城市学院医学院,浙江杭州310015 [2]浙江大学药学院,浙江杭州310018 [3]浙江大学理学院,浙江杭州310018

出　　处：《浙江大学学报（医学版）》2014年第2期175-179,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家科技支撑计划(2012BAI31B08)

摘　　要：目的：提高沙地柏多酚的纯化程度并考察其抗氧化活性。方法：采用Folin-Ciocalteu法测定沙地柏的多酚含量，经筛选用HPD-700大孔树脂纯化沙地柏多酚。通过对沙地柏多酚，1，1-二苯基-2-三硝基苯肼（ DPPH）自由基、超氧阴离子、羟自由基的清除能力、总抗氧化能力及三价铁离子（ Fe3＋）还原能力的测定来评价其抗氧化能力。结果：经纯化后，沙地柏多酚纯度由0．53‰提高到9．95‰。抗氧化活性测定结果表明，沙地柏多酚对DPPH自由基的抑制率和Fe3＋还原能力的维生素C当量质量浓度分别为9．4μg/ml、30．09μg/ml，对超氧阴离子和羟自由基的清除能力分别为151．83 U/ml、204．59 U/ml，总抗氧化能力为72．68 U/ml。结论：沙地柏多酚具有较强的抗氧化活性，值得进一步研究其药理作用。Objective：To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties .Methods： Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700 , and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry .The antioxidant properties of polyphenols were evaluated by total antioxidant capacity （ T-AOC ） and its activeties of scavenging＆nbsp;DPPH （1,1diphenyl-2-picry-hydrazyl） radicals, superoxide anion （ O2 · -）, hydroxyl free radicals （OH· ） and ferric reducing antioxidant power （FRAP）.Results： After purification, the purity of polyphenols increased from 0.53‰ to 9.95‰.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml.Its scavenging capacity for superoxide anion （ O2 · -） and hydroxyl free radicals （ OH · ） was 151.83 U/ml and 204.59 U/ml.The total antioxidant capacity was 72.68 U/ml.Conclusion：Polyphenols from Sabina vulgaris Antoine have high antioxidant properties , suggesting that it worth further study of its pharmacological effects .

关 键 词：柏科 化学 酚类 抗氧化剂 

分 类 号：R284[医药卫生—中药学]