转化生长因子β1及其受体与高碘促成纤维细胞增殖作用的相关性研究  

The effect of TGF-β1, TGF-βRI and high concentration iodine in the promotion of fibroblast proliferation: correlation study

作　　者：华浅近[1] 祖茂衡[1] 胡琳[1] 庄银苹[2] 

机构地区：[1]徐州医学院附属医院介入放射科,221002 [2]徐州医学院医学影像学院布一加综合征实验室

出　　处：《介入放射学杂志》2014年第5期431-434,共4页Journal of Interventional Radiology

基　　金：江苏省科技创新与成果转化专项基金资助项目(BL2012021);徐州医学院研究生科技创新工程研究项目(XYLC-1220)

摘　　要：目的研究转化生长因子β1(TGF-β1)及其受体Ⅰ(TGF-βRⅠ)与高碘促成纤维细胞增殖作用的相关性,探索布-加综合征(BCS)隔膜组织形成机制。方法实验分为5组,空白对照组、溶媒组、KI组、TGF-βRⅠ抑制剂(SD-208)组和SD-208和碘化钾(KI)共同作用组。采用CCK-8法检测TGF-βRⅠ抑制剂对高碘培养环境中成纤维细胞增殖率的影响;采用免疫印迹法检测不同浓度(0、250、500、1 000、2 000、3 000μg/L)碘离子对成纤维细胞TGF-β1、TGF-βRⅠ蛋白表达的影响。结果①在1 000μg/L碘培养环境中,KI与SD-208共同作用组成纤维细胞增殖率(1.29±0.41)高于SD-208组(0.52±0.10),而低于KI组(1.70±0.03),差异有统计学意义(P<0.05)。②1 000μg/L和2 000μg/L高碘组成纤维细胞TGF-β1蛋白相对表达量高于其他各组(P<0.05)。各组间成纤维细胞TGF-βRⅠ蛋白相对表达量差异无统计学意义(P>0.05)。结论①高碘因素可能通过提高成纤维细胞TGF-β1蛋白表达而促进成纤维细胞增殖;②高碘导致的成纤维细胞增殖可能与BCS隔膜形成相关。Objective To study the relationship between transforming growth factor-β1 （TGF-β1）, transforming growth factor-β receptor I （TGF-13R I ） and high concentration iodine in promoting fibroblast proliferation so as to explore the pathogenesis of the membranous formation in Budd- Chiari syndrome. Methods The experiment included five groups： blank control group, solvent group, KI group, TGF-βR I inhibitor group （SD-208） and SD-208 plus KI combination group.① Fibroblasts were cultured in high content of iodine and treated with TGF-βRI inhibitor then the fibroblast proliferation activity was determined by CCK-8 assy. ② The protein expressions of TGF-β1 and TGF-βR I of fibroblasts in different concentrations of iodine （0, 250,500, 1 000, 2 000 and 3 000 μg/L） were determined by Western-blot method. Results ①When the culture solution was of 1 000 μg/L iodine concentration, the cell proliferation rate of the SD-208 plus KI combination group （A ：1.29±0.41 ） was significantly higher than that of the control group （0.52 ± 0.10）, but significantly lower than that of the KI group （1.70 ± 0.03） with P 〈 0.05. ②Fibroblast TGF-β1 protein relative expression levels in the groups with the iodine concentration of 1 000 μg/L and 2 000 μg/Lwere significantly higher than those of the other groups （P 〈 0.05）. No significant difference in fibroblast TGF- βR I protein relative expressions existed between each other groups （P 〉 0.05）. Conclusion ① High concentration of iodine may promote the proliferation of fibroblasts through raising TGF - β1 protein expression. ②Theproliferation of fibroblasts caused by high concentration of iodine may be related to the membranous formation in Budd-Chiari syndrome.（J Intervent Radiol, 2014, 23： 431-434）

关 键 词：布-加综合征 成纤维细胞 碘 细胞因子类 隔膜阻塞 

分 类 号：R575[医药卫生—消化系统]