刺参养殖池塘水体微生物群落功能多样性的季节变化  

Seasonal variation of functional diversity of aquatic microbial community in Apostichopus japonicus cultural pond

作　　者：闫法军[1,2] 田相利[1] 董双林[1] 阳钢[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]山东省淡水渔业研究院,济南250013

出　　处：《应用生态学报》2014年第5期1499-1505,共7页Chinese Journal of Applied Ecology

基　　金：教育部新世纪优秀人才计划项目(NCET-08-0503);山东省杰出青年科学基金项目(JQ201009);"十二五"国家支撑计划项目(2011BAD13B03);青岛市关键技术重大攻关计划项目(12-6-1-6-hy)资助

摘　　要：利用Biolog技术和冗余分析(RDA)方法对刺参养殖池塘水体微生物群落功能多样性的季节变化及其与环境因子间的关系进行研究.结果表明:刺参池塘水体微生物对碳源总量和单类碳源的利用均具有显著的季节变化,总体表现为夏季最高、冬季最低,其利用比例较高的碳源类型为聚合物.主成分分析表明,刺参池塘水体微生物群落碳代谢方式具有显著的季节变化,其中,与主成分1显著相关的碳源主要有10种,分别属于聚合物、糖类、羧酸、氨基酸和胺.刺参池塘水体微生物碳代谢多样性指数Shannon、McIntosh、Simpson和S-E均匀度均存在显著的季节变化,但不同指数之间的变化有较大差异.RDA分析表明,TP、NO3--N、TN和PO43--P是影响刺参池塘水体微生物群落功能多样性季节变化的主要因素.The functional diversity of aquatic microbial communities in sea cucumber （Apostwhopus japonicus） cultural ponds was examined in this paper. The Biolog plate technique and redundancy analysis （RDA） method were used to evaluate seasonal changes and their relationships with environmental factors. The results showed that both total amount and types of carbon sources utilized by microbes in the sea cucumber cultural ponds varied seasonally, and were the highest in summer and lowest in winter, with polymers being the main type of carbon sources. Principal component analysis revealed that the carbon utilization diversity of the microbial communities varied significantly over the seasonal courses. A total of 10 categories of carbon sources were significantly related to the principal component 1, among which were polymers, carbohydrates, carboxylic acids, amino acids, and amines. Significant seasonal changes were detected for all carbon utilization diversity indices of the microbial communities, including Shannon, McIntosh, Simpson, and S-E. However, seasonal variations were different among the microbial diversity indices. RDA analysis revealed that TP, NO3-N, TN, and PO43-P were the critical environmental factors influencing the seasonal changes in functional diversity of aquatic microbial community in sea cucmnber cultural ponds.

关 键 词：微生物群落 功能多样性 季节变化 BIOLOG 冗余分析 环境因子 刺参池塘 

分 类 号：S917.1[农业科学—水产科学]