小鼠柯萨奇病毒性心肌炎模型的优化  

The optimization of viral infection dose in Coxsackievirus-induced murine model of viral myocarditis

作　　者：熊飞[1] 万方方[1] 钱乾[1] 徐薇[1] 

机构地区：[1]苏州大学生物医学研究院,苏州215123

出　　处：《现代免疫学》2014年第3期183-189,共7页Current Immunology

基　　金：国家自然科学基金(31270973)

摘　　要：在两种品系小鼠中建立、优化和规范B组3型柯萨奇病毒(CVB3)诱导的病毒性心肌炎小鼠模型。首先以100TCID50-10 000TCID50之间剂量的CVB3Nancy株病毒经腹腔感染易感BALB/c雄性小鼠,通过体重减轻率、死亡率和心脏病理及心肌CK-MB水平,评估建立最佳病毒性心肌炎的最佳剂量;而后摸索了在C57BL/6小鼠建立病毒性心肌炎的CVB3剂量。通过精细比较发现雄性BALB/c小鼠致心肌炎的最佳CVB3病毒滴度数量级在1000TCID50;进一步确定1500TCID50是在雄性BALB/c小鼠中诱导病毒性心肌炎的最佳剂量;C57BL/6小鼠不是易感小鼠,但在1500TCID50CVB3感染下也可诱导轻微可见的病毒性心肌炎,便于在多数基因敲除鼠内的病毒性心肌炎研究。本研究为规范病毒性心肌炎模型的建立、为后续病毒性心肌炎机制的深入研究奠定了重要的基础模型。To optimize the viral dose to establish Coxsackievirus B3 （CVB3）-induced myocarditis murine model in different strains of mice, 100TCID50 to 10 000TC1D50 doses of CVB3 were intraperitoneally injected into sensitive BALB/c male mice and the non-sensitive C57BL/6 mice. The severity of viral myocarditis was assessed by evaluation of weight loss, mortality, myo- cardial CK-MB level and cardiac pathology. 1500TCID50 of CVB3 was confirmed to be the best dosage to induce viral myocar- ditis in sensitive BALB/c male mice; and this virus dose also induced a much weaker but visible myocarditis in C57BL/6 mice.Taken together, 1500TCID50 dosage of CVB3 was demonstrated to be the best modebestablishing dosage in BALB/c mice. Al- though C57BL/6 mice is not a recommended strain for CVB3, 1500TCID50 of CVB3 still could induce myocarditis in these mice which may facilitate the research of viral myocarditis in most gene-knockout mice. Our data provide a very fundamental and key standard for the establishment of CVB3-viral myocarditis and facilitate the future study of this disease.

关 键 词：CVB3 病毒性心肌炎 剂量 品系 

分 类 号：R593.12[医药卫生—内科学]