吲哚青绿介导光动力抑制兔眼后发性白内障的实验研究  

Inhibitory Effect of Photodynamic Therapy Mediated by Indocyanine Green on Posterior Capsule Opacification in Rabbits

作　　者：查旭[1] 李兰[2] 张远平[1] 曹倩[2] 李云川[2] 赵学英[1] 

机构地区：[1]昆明医科大学第二附属医院眼科,云南昆明650101 [2]昆明市第一人民医院眼科,云南昆明650034

出　　处：《昆明医科大学学报》2014年第5期46-48,共3页Journal of Kunming Medical University

基　　金：昆明市科技局基金资助项目(08S090207);云南省科技厅-昆明医科大学联合专项基金资助项目(2012FB056);云南省教育厅科研基金资助项目(2012C016)

摘　　要：目的研究吲哚青绿(indocyanine green,ICG)介导光动力对兔眼后发性白内障发生的抑制作用.方法 32只兔眼随机分为对照组、低剂量组、中剂量组、高剂量组,分别行晶状体超声乳化及皮质吸出术,低、中、高剂量组再皮质吸出后前房内注入0.5 mL浓度分别为1.25 mg/mL,2.5 mg/mL,5 mg/mL的ICG后用810 nm的半导体激光对囊膜进行低能量(50 mW/cm2)照射2 min.术后3个月时观察后囊膜的混浊程度及囊膜中晶状体上皮细胞增生细胞核抗原的表达.结果中高剂量组晶状体后囊膜混浊程度EPCO值呈剂量依赖性的下降,组间比较P<0.01,差异有统计学意义;囊膜的晶状体上皮细胞增殖核抗原增殖指数呈剂量依赖性的下降,各组间比较P<0.01,差异有统计学意义.结论 ICG的光动力作用在兔眼可降低术后3个月时后囊膜混浊的程度,其作用是通过术中ICG的光动力毒性清除了囊袋内的晶状体上皮细胞,从而减少了晶状体上皮细胞的增殖程度.Objective The purpose of this study was to research the inhibitory effect of photodynamic therapy （PDT） mediated by indocyanine green （ICG） on posterior capsule opacification （PCO）.Methods Thirty-two eyes of rabbits were randomly divided in 4 groups：the control,low-,middle-and high-dose groups.Extracapsular lens extraction by phacoemulsification was performed on 32 eyes.0.5 mL ICG of 1.25 mg/mL,2.5 mg/mL and 5 mg/mL was injected into the capsular bag of the eyes in the low-,middle-and high-dose groups respectively,and the eyes were illuminated with a diode laser （wavelength 810 nm） for 2 min.At 3 months after surgery,the degrees of PCO and the expressions of proliferating cell nuclear antigen （PCNA） in lens epithelial cells were evaluated.Results After surgery with ICG-mediated PDT,the degrees of PCO have decreased in a dose-dependent manner in the middle-and high-dose groups.There were statistically significant differences among groups （P ＜ 0.01）.The proliferation indexes of PCNA in lens epithelial cells were reduced in a dose-dependent manner,with the significant differences among groups （P ＜ 0.01） Conclusion ICG-mediated PDT could reduce the degree of PCO in the eyes of rabbits,which was due to the photodynamic toxicity of ICG by removing lens epithelial cells from the capsular bag and reducing the cells proliferation.

关 键 词：吲哚青绿 光动力作用 后发性白内障 

分 类 号：R776.1[医药卫生—眼科]