95%乙醇在去除LASIK角膜板层刀片残留物质中的应用  

Application of removing residual substance of microkeratomeblade with 95% alcohol

作　　者：梁歌[1] 刘海灵[2] 高维平[1] 李鹏飞[3] 姜严明[1] 

机构地区：[1]第二炮兵总医院眼科,北京100088 [2]北京师范大学分析测试中心 [3]北京朝阳医院药事部

出　　处：《中国中医眼科杂志》2014年第2期136-139,共4页China Journal of Chinese Ophthalmology

摘　　要：目的验证95%乙醇(体积分数)反复擦拭板层刀片,再用无菌蒸馏水冲洗,能有效去除其上残留物质,降低准分子激光原位角膜磨镶术(LASIK)后弥漫性板层角膜炎(DLK)的发生率。方法采用色谱纯乙腈浸泡并超声提取刀片上的残留物,对提取液和空白试剂乙腈分别进行30 min的色谱柱梯度分离并进行高分辨质谱分析;术后病人随访1年以上,观察角膜情况。结果处理后的刀片均未检出残留物质;所有随访患者术后角膜情况良好。结论 95%乙醇反复擦拭板层刀片,再用无菌蒸馏水冲洗,可有效去除角膜板层刀残留物质,防止DLK的发生。OBJECTIVE To verify the effectiveness of the method that microkeratomeblades wiped with 95% alcohol and rinsed with sterile distilled water may remove residual substance of mierokeratomes that can cause diffuse lamellar keratitis （DLK）. METHODS Extracted residual substance on the blade by using pure acetonitrile soaking and ultrasonic extraction chromatography, analyzed reagent blank acetonitrile respectively for 30 min gradient of chromatographic column separation and high resolution mass spectrometry. Postoperative patients were followed up for more than a year to observe the corneal situation. RESULTS No compounds on the tested microkeratomeblades had been detected after this treatment, and all the patients were in good postoperative condition. CONCLUSIONS Microkeratomeblades wiped with 95% alcohol repeatedly and rinsed with sterile distilled water could effectively remove the residual substance and prevent the happening of DLK.

关 键 词：角膜板层刀 质谱分析 弥漫性板层角膜炎 

分 类 号：R778.1[医药卫生—眼科]