超声检测肝癌血流分级及其与HIF-1α、MVD表达的关系  

Relationship between blood flow assessed by ultrasound, MVD and expression of HIF-1α in hepatocellular carcinoma

作　　者：张璟卓 刘颖[3] 马艳艳[1,2] 

机构地区：[1]辽宁省大连医科大学附属第二医院超声科,辽宁省大连市116023 [2]大连医科大学研究生院,辽宁省大连市116023 [3]大连医科大学附属第二医院体检中心,辽宁省大连市116023

出　　处：《世界华人消化杂志》2014年第11期1587-1591,共5页World Chinese Journal of Digestology

摘　　要：目的:超声观察肝癌性质及其血流情况,并探讨缺氧诱导因子-1α(hypoxia inducible factor-1alpha,HIF-1α)与微血管密度(microvessel density,MVD)在肝癌组织中的表达情况及二者间关系.方法:对60例经病理证实的原发性肝癌手术切除术前进行二维及彩色多普勒超声检查,进行血流分级,通过实验室免疫组织化学的方法检测HIF-1α、MVD在肝癌组织中的表达.结果:血流等级和HIF-1α、MVD相关,在肝癌组织中当血流分级为Ⅲ级时HIF-1α、MVD表达率最高,分别为81.6%(92.61%±3.10%)、80.1%(85.16%±2.33%),在正常肝组织中HIF-1a、MVD表达率分别为12.28%±7.21%,为11.33%±7.21%,二者比较具有显著性差异(P<0.05).HIF-1α的表达与肝癌新生血管的生成密切相关,MVD高表达、HIF-1α表达越高,彩色多普勒显示血流越丰富.结论:彩色多普勒超声结合上述指标可从不同方面反映肝癌血管生成的特性,有利于对肝癌血管生成进行评估.AIM： To investigate the ultrasonic signs of hepatocellular carcinoma （HCC）, the expression of hypoxia inducible factor-1 alpha （HIF-1α） and microvessel density （MVD）, and to analyze their correlations. METHODS： Sixty patients who underwent duplex sonography and color Doppler ultrasonography before surgery and were surgically proved to have HCC were included. Immunohistochemical method was used to detect the expression of HIF-1α and MVD in HCC.RESULTS： There was a correlation between blood flow grade and MVD. In liver cancer tissues when blood flow classification was grade Ⅲ, the rates of HIF-1α and MVD expression were highest [81.6% （92.61% ± 3.10%）, 80.1% （85.16% ± 2.33%）], significantly higher than those in normal liver tissues （12.28% ± 7.21%, 11.33%±7.21%, P 〈 0.05 for both）. The expression of HIF-1α is related closely to hepatocellular carcinoma angiogenesis; higher expression of MVD was associated with higher expression of HIF-1α and more abundant blood flow. CONCLUSION： Combination of color Doppler flow imaging and immunohistochemical parameters is useful for evaluating the angiogensis of HCC.

关 键 词：肝细胞癌 微血管密度 缺氧诱导因子-1Α 超声检查 

分 类 号：R735.7[医药卫生—肿瘤] R445.1[医药卫生—临床医学]