SCUBE3对骨肉瘤细胞功能的影响及其与预后的关系  

Role of SCUBE3 in promoting osteosarcoma cell growth and its association with prognosis

作　　者：宋国徽[1] 王晋[1] 卢金昌[1] 徐怀远[1] 赵志强[1] 唐清连[1] 邹昌业[1] 尹军强[1] 谢显彪[1] 沈靖南[1] 

机构地区：[1]中山大学附属第一医院骨肿瘤科,广东广州510080

出　　处：《南方医科大学学报》2014年第5期617-621,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81272940;81072193);中山大学青年教师重点培育基金(13yk2d08)~~

摘　　要：目的探讨SCUBE3在骨肉瘤细胞和组织中的表达及其与患者预后的关系。方法利用Western blotting技术检测5种骨肉瘤细胞株SCUBE3的表达,利用化学合成siRNA转染骨肉瘤细胞,靶向抑制SCUBE3表达。CCK8细胞增殖实验观察SCUBE3对U2OS细胞增殖的影响,流式细胞仪检测细胞周期改变。免疫组化检测SCUBE3在骨肉瘤组织中的表达,并运用Kaplan-Meier法进行生存分析。结果 SCUBE3在骨肉瘤细胞株中的表达均高于正常成骨细胞。干扰SCUBE3表达能抑制U2OS的生长(P<0.05),同时细胞周期阻滞在G1期。Kaplan-Meier生存分析显示,SCUBE3高表达的患者预后比低表达组差(P=0.036)。结论 SCUBE3在骨肉瘤中高表达,它能促进骨肉瘤细胞的增殖并与患者不良预后相关,提示其可能成为潜在的治疗靶点。Objective To detect the expression of SCUBE3 in human osteosarcoma cell lines and surgical specimens of osteosarcomas and investigate its association with the patients＆#39; prognosis. Methods The expression of SCUBE3 protein was detected in 5 osteosarcoma cell lines using Western blotting. CCK8 assay was used to assess the effect of SCUBE3 suppression mediated by two specific small interfering RNAs （siRNAs） on the proliferation of U2OS osteosarcoma cells, and the cell cycle changes were detected using flow cytometry. Immunohistochemistry was performed to detect the expression of SCUBE3 in 60 osteosarcoma tissues, and Kaplan-Meier method was performed for survival analysis of the patients. Results Compared with osteoblast hFOB1.19 cells, the osteosarcoma cell lines all showed significantly higher expressions of SCUBE3. In U2OS cells, suppression of SCUBE3 expression by siRNA significantly inhibited the cell proliferation （P＆lt;0.05）. Kaplan-Meier survival analysis indicated that patients with high SCUBE3 expression had worse prognosis than those with low SCUBE3 expression （P=0.036）. Conclusion SCUBE3 is up-regulated in the 5 osteosarcoma cell lines and in primary osteosarcoma tissues to promote the proliferation of osteosarcoma cells. A high SCUBE3 expression in osteosarcoma tissues is associated with a poor prognosis of the patients, suggesting that SCUBE3 can serve as a potential therapeutic target for osteosarcoma.

关 键 词：SCUBE3 骨肉瘤 细胞增殖 RNA干扰 

分 类 号：R738.1[医药卫生—肿瘤]