康脑液对局灶性脑缺血再灌注大鼠Pi3k mRNA与Akt mRNA的影响  

Effects of Kangnao Liquid on Expressions of Pi3k mRNA and Akt mRNA in Focal Cerebral Ischemic-Reperfusion Injury in Rats

作　　者：肖志娟[1] 赵志敏[1] 邹玉安[2] 薛茜[2] 邢立强[3] 

机构地区：[1]河北北方学院,075000 [2]河北北方学院附属第一医院神经内科 [3]河北北方学院生命科学研究中心

出　　处：《天津医药》2014年第5期436-439,共4页Tianjin Medical Journal

基　　金：河北省卫生厅课题资助项目(项目编号:20090591)

摘　　要：目的观察康脑液对SD大鼠脑缺血再灌注(I/R)损伤的治疗效果并研究其对I/R大鼠Pi3k mRNA及Akt mRNA的影响。方法 180只雄性SD大鼠随机分为6组:假手术组,模型组,康脑液高、中、低剂量组和尼莫地平组。药物治疗组分别给予康脑液24、12、6 g/(kg·d),尼莫地平1 mg/(kg·d),每天灌胃1次,其他组每天给予等量蒸馏水,给药7 d后,除假手术组外,线栓法制备大鼠右侧大脑中动脉闭塞模型。大鼠缺血2 h,于再灌注24 h后观察各组神经功能,再灌注12、24、48、72、168 h原位杂交技术观察各组脑组织Pi3k mRNA、Akt mRNA表达。结果与模型组相比,康脑液各剂量组神经功能明显改善,Pi3k mRNA及Akt mRNA的表达明显高于模型组,尼莫地平组Pi3k mRNA及Akt mRNA的表达也明显高于模型组,但较康脑液高、中剂量组低。结论康脑液可能通过增加脑缺血再灌注大鼠缺血周围区域Pi3k mRNA及Akt mRNA的表达,从而实现其对脑组织的保护作用。Objective To observe the therapeutically effect of kangnao liquid on Pi3k mRNA and Aktm RNA ex-pressions in rats with focal cerebral ischemia-reperfusion （I/R） injury. Methods 180 male SD rats were randomly divided into 6 groups：sham operated group, model group, three kangnao liquid groups （high-dose, medium-dose and low-dose） and nimodipine group. Rats in kangnao liquid groups were administrated with kangnao liquid of 24 g/（kg ＆#183; d）, 12 g/（kg ＆#183; d） and 6 g/（kg ＆#183; d）, orally once a day. Rats in nimodipine group were given nimodipine 1 mg/（kg ＆#183; d）. Rats in model group and sham group were treated with the same volume of distilled water for 7 days. The animal model of middle cerebral artery occlusion （MCAO） was established by a monofilament method from right internal carotid artery. The neurological evaluation was per-formed 24 h after reperfusion. The in situ hybridization was used to investigate the expression levels of Pi3k mRNA and Akt mRNA in rats on 12 h, 24 h, 48 h, 72 h and 168 h after ischemia for 2 h. Results Compared with model group, neurological functions were improved significantly in kangnao liquid groups. The expression levels of Pi3k mRNA and Akt mRNA were al-so significantly higher in kangnao liquid groups than those of model group. The expression levels of Pi3k mRNA and Akt mRNA were significantly higher in nimodipine group than those of model group, but which were lower compared with those of high-dose and medium-dose kangnao liquid groups. Conclusion Kangnao liquid can protect nerve cells by enhancing the expressions of Pi3k mRNA and Akt mRNA in rats with cerebral ischemia-reprefusion injury.

关 键 词：再灌注损伤 脑缺血 1-磷脂酰肌醇3-激酶 原位杂交 康脑液 PI3K AKT通路 

分 类 号：R743[医药卫生—神经病学与精神病学]