曲古菌素A作用胃癌细胞SGC-7901后GDF-15的表达及其在胃腺癌组织中的表达  

Expression of GDF-15 in trichostatin A-treated gastric cancer SGC-7901 cells and in gastric adenocarcinoma tissues

作　　者：李云龙[1] 崔武[1] 李晓林[1] 王浩[1] 田瑜[1] 曹志刚[1] 佟柏峰[1] 邹小明[1] 

机构地区：[1]哈尔滨医科大学附属二院普外科,黑龙江省哈尔滨市150080

出　　处：《世界华人消化杂志》2014年第10期1391-1395,共5页World Chinese Journal of Digestology

基　　金：教育部高等学校博士学科点专项科研基金联合资助项目;No.20102307120014;哈尔滨市科技创新人才研究专项基金资助项目;No.2009RFQX016~~

摘　　要：目的:研究生长分化因子-15(growth differ-entiation factor-15,GDF-15)基因在曲古菌素A(trichostatin A,TSA)作用胃癌细胞系SGC-7901后及胃腺癌组织中的表达.方法:采用Real-time PCR检测胃癌细胞系SGC-7901经过TSA干预后GDF-15基因表达的变化,免疫组织化学检测GDF-15基因在胃癌及癌旁正常组织中的表达.结果:GDF-15基因在胃癌细胞系SGC-7901经75 ng/mL TSA作用48 h后,表达下调,差异具有显著性(P<0.05),免疫组织化学显示胃腺癌组织中GDF-15基因表达明显高于周围正常胃组织(P<0.05).结论:GDF-15基因表达下降可能在TSA促胃癌细胞系SGC-7901凋亡中发挥作用.GDF-15基因在胃腺癌组织中表达增高.AIM： To examine the expression of growth differentiation factor-15 （GDF-15） in gastric cancer SGC-7901 cells treated with TSA and in gastric carcinoma tissues. METHODS： GDF-15 gene expression in gastric cancer cell line SGC-7901 after TSA treatment was detected by real-time PCR, and GDF-15 protein expression in gastric cancer and tumor-adjacent normal tissues was detected by immunohistochemistry. RESULTS： GDF-15 gene expression was significantly down-regulated in gastric cancer cell line SGC-7901 treated with 75 ng/mL TSA for 48 h （P 〈 0.05）. Immunohistochemistry analysis demon-strated that GDF-15 protein expression in gastric adenocarcinoma was significantly higher than that in tumor-adjacent gastric tissue （P 〈 0.05）. CONCLUSION： Down-regulation of GDF-15 gene expression may play a role in TSA-induced apoptosis of SGC-7901 cells. GDF-15 protein ex- pression is increased in gastric carcinoma.

关 键 词：生长分化因子-15 胃癌 曲古菌素A SGC-7901 

分 类 号：R735.2[医药卫生—肿瘤]