鼻腔鼻窦内翻性乳头状瘤中PTEN和缺氧诱导因子1α的表达及意义  

Expression and significance of PTEN and HIF-1αproteins in sinonasal inverted papilloma

作　　者：张文[1] 温树信[2] 张涛源[2] 王斌全[2] 高伟[2] 李丽娟[2] 

机构地区：[1] 山西医科大学第一临床医学院耳鼻咽喉头颈外科, 太原030001 [2] 山西医科大学第一医院耳鼻咽喉头颈外科

出　　处：《中华耳鼻咽喉头颈外科杂志》2014年第5期399-403,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的：探讨与张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶基因（ phosphatase and tensin homolog deleted from chromosome ten ， PTEN ）、缺氧诱导因子1α（ hypoxia-inducible factor 1α， HIF-1α）在不同病理分级的鼻腔鼻窦内翻性乳头状瘤（ sinonasal inverted papillomas，SNIP）组织中的表达及意义。方法选取55例SNIP患者的资料，按不同病理分级分为无不典型增生组30例，不典型增生组11例，恶性变组14例。鼻中隔偏曲患者10例作为对照（NM）组。用免疫组化SP法检测SNIP患者乳头状瘤组织和鼻中隔偏曲患者下鼻甲黏膜组织中PTEN和HIF-1α蛋白的表达，分析二者在NM与SNIP之间、不同病理分级的SNIP之间的表达差异，以及PTEN和HIF-1α蛋白的表达与肿瘤复发间的相关性。以SPSS 16.0软件进行统计学分析。结果 PTEN蛋白在NM和SNIP组的阳性率分别为100％、65.5％，差异有统计学意义（U＝147，P＝0.014），HIF-1α蛋白在NM组无表达，在SNIP组的表达率为30.9％，差异有统计学意义（ U＝190，P＝0.045）。在无不典型增生组、不典型增生组、恶性变组中PTEN蛋白的阳性率分别为83.3％、63.6％、28.6％，三组差异有统计学意义（H ＝12.644，P ＝0.002），HIF-1α蛋白在三组的阳性率分别为16.7％、45.5％、50.0％，三组差异有统计学意义（H＝8.292，P＝0.016）。在SNIP组中，PTEN和HIF-1α蛋白的表达呈负相关（r＝-0.503，P＝0.001）。术后随访20～84个月发现，55例患者中22例复发，在术后复发和未复发组中，PTEN蛋白的阳性表达率分别为45.5％、82.8％，差异有统计学意义（χ2＝7.834，P＝0.005）；在术后复发和未复发组中HIF-1α蛋白阳性表达率分别为36.4％、24.1％，差异无统计学意义（χ2＝0.901，P＝0.343）。结论随着SNIP恶性程度的增加，PTEN蛋白表达减少，HIF-1α蛋白表达增加；PTEN和HIF-1α可能共同参与了SNIP的进展及恶�Objective To investigate the expression and clinical significance of phosphatase and tensin homology deleted on ehromosome ten （ PTEN ） and hypoxia-inducible factor 1 alpha （ HIF-1α） in sinonasal inverted papilloma （SNIP） of different pathological grades.Methods Fifty-five paraffin samples from patients with SNIP and a control group of 10 paraffin samples of patients with nomal nasal cavity mucosa （NM） who underwent inferior turbinectomy were consisted in this study.Among the 55 cases of SNIP, 30 cases were without dysplasia subtypes , 11 cases were with dysplasia subtypes , and 14 cases with canceration to squamous cell carcinoma （ SCC ） subtypes.PTEN and HIF-1αexpression in SNIP was detected by immunohistochemistry.The differences between NM and SNIP , and among the three subtypes were analyzed , and the relationship between PTEN , HIF-1αexpression and SNIP recurrence and the correlation between PTEN expression and HIF-1αexpression were also analyzed.SPSS 16.0 software was＆amp;nbsp;used to analyze the data.Results The positive expression rate of PTEN in NM and SNIP was 100% and 65.5%, the difference was significant （ U =147, P =0.014）, while HIF-1αwas 0 and 30.9%, the difference was significant （U=190, P=0.045）.The positive expression rate of PTEN in SNIP without dysplasia, SNIP with dysplasia and NSCC was 83.3%, 63.6%, 28.6%, respectively, the difference was significant（H =12.644, P =0.002）; while HIF-1αwas 16.7%, 45.5%, 50.0%, respectively, the difference was significant（H=8.292, P=0.016）.A total of 22 SNIP patients recurred.PTEN had lower expression in recurrent SNIP （ 45.5%） than that in non-recurrent SNIP （ 82.8%） , and the difference was significant（χ2 =7.834, P =0.005）.However, the expression of HIF-1αhad no significant difference between recurrent SNIP and the SNIP which had no recurrence （χ2 =0.901, P=0.343）.The expression of PTEN protein was negatively correlated with that of HIF-1αprotein （r=-0.503, P=0.001）.Conclusions PTEN expressi

关 键 词：乳头状瘤 内翻 PTEN 磷酸水解酶 缺氧诱导因子1 Α亚基 恶性变 肿瘤复发 局部 

分 类 号：R739.62[医药卫生—肿瘤]