GABAA受体与醒脑静注射液抗神经细胞凋亡作用的相关性  

The Effect of GABAA Receptor and Xingnaojing Injection on Resisting Neurocyte Apoptosis：A Correlation Study

作　　者：曾玲[1,3] 王海宽[2] 

机构地区：[1]广州中医药大学基础医学院,广东广州510405 [2]广州中医药大学针灸推拿学院,广东广州510405 [3]阿拉巴马州伯明翰大学营养学系,美国阿拉巴马州35205

出　　处：《按摩与康复医学》2014年第5期25-28,共4页Chinese Manipulation and Rehabilitation Medicine

摘　　要：目的：研究GABAA受体是否参与醒脑静注射液抗神经细胞凋亡的作用。方法：采用血清饥饿3天的方法建立PC12细胞凋亡模型，并随机分为正常对照组（control组）、模型组（model组）、醒脑静组（XNJ组），GABAA受体阻断剂组（BIC组）、醒脑静加GABAA受体阻断剂组（XNJ＋BIC组）。正常对照组以含10%FBS的正常培养基培养，不作任何处理；模型组换成无血清培养基培养；醒脑静组在无血清培养基基础上加入1.5μL/mL醒脑静注射液；BIC组加入终浓度为200μmol/mL的荷包牡丹碱；XNJ＋BIC组同时加入醒脑静注射液1.5μL/mL和终浓度为200μmol/mL的荷包牡丹碱；每组5个复孔，采用Annexin V/PI双染流式细胞仪检测神经细胞凋亡数，用Western Blotting检测GABAA受体的蛋白表达。结果：XNJ＋BIC组凋亡少于模型组（P〈0.05）；醒脑静注射液加入GABAA受体阻断剂后其GABAA受体蛋白表达较醒脑静注射液降低。结论：醒脑静注射液抗细胞凋亡的作用机制与提高GABAA受体从而抑制凋亡效应有关。Objective：To elucidate the role of GABAA receptor in the protective effect of xingnaojing injection on neurons apoptosis. Methods：The PC12 apoptosis model was established by serum starvation for 3 days. The cells were randomly classified into four groups： control group, model group, xingnaojing group （XNJ group）, GABAA receptor antagonist Group （BIC group）, xingnaojin and GABAA receptor antagonist Group （XNJ＋BIC group）. The control group only cultured with 10%FBS of normal medium;model group treated with serum-free culturel;XNJ group joined for 1.5μL/mL xingnaojing injection after the serum-free culturel. BIC Group joined 200μmoL/mL bicuculline;XNJ＋BIC group joined 1.5μL/mL xing-naojing injection and 200μmoL/mL bicuculline. Each group has 5 double wells. Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry （FCM）, and Western blot was applied to detect the expression of GABAA receptor protein. Results：XNJ＋BIC group compared with model group, the apoptosis is significantly decreased （P〈0.05）. The expression of GABAA is significantly decreased by the combination of xingnao-jing injection and GABAA receptor antagonist bicuculline. Conclusion：The mechanisms of xingnaojing injection protection against cell apoptosis are related to the anti-apoptosis effect of GABAA receptor.

关 键 词：醒脑静注射液 PC12细胞 凋亡 GABAA受体 相关性 

分 类 号：R-33[医药卫生]