miR-24-2对人骨肉瘤细胞系U-2OS体外增殖能力的影响  

Effect of miR-24-2 on Proliferation of U-2OS Cell Line

作　　者：梁德勇[1] 陈升[2] 王野[2] 富伟能[2] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学基础医学院医学遗传学教研室,沈阳110001

出　　处：《中国医科大学学报》2014年第5期393-395,共3页Journal of China Medical University

基　　金：国家自然科学基金(81172577);辽宁省教育厅高校科研计划(L2010595)

摘　　要：目的探讨miR-24-2对人骨肉瘤细胞系U-2OS体外增殖能力的影响。方法用脂质体法将miR-24-2模拟物转染入人骨肉瘤细胞系U-2OS。将U-2OS细胞分为miR-24-2模拟物转染组、miR-24-2抑制剂转染组、阴性对照组和正常对照组4组。转染后采用实时定量RT-PCR检测各组细胞miR-24-2的表达水平,采用MTT法检测各组细胞的增殖情况。结果与阴性对照组和正常对照组比较,miR-24-2模拟物转染组细胞增殖能力显著下降,而miR-24-2抑制剂转染组细胞增殖能力显著增强。结论 miR-24-2能够抑制人骨肉瘤细胞系U-2OS的体外增殖能力,可望成为骨肉瘤治疗的分子标志物。Objective To explore the effect of miR-24-2 on the in vitro proliferation of U-2OS cells. Methods U-2OS cells were randomly allocated into 4 groups：miR-24-2 mimic group,miR-24-2 inhibitor group,negative control group,and normal control group. MicroRNAs were transfected into U-2OS cells using Lipofectamine？ 2000. miR-24-2 expression level and the proliferation of U-2OS cells after transfection were detected by real-time quantitative RT-PCR（RT-qPCR）and MTT proliferation assays,respectively. Results RT-qPCR results showed that miR-24-2 level was significantly higher in the miR-24-2 mimic group and lower in the miR-24-2 inhibitor group than those in the controls,indicating the successive transfection. MTT proliferation assay results proved that the cell viability was significantly lower in the U-2OS cells transfected with miR-24-2 mimic and higher in inhibitor groups compared to the control. Conclusion MiR-24-2 inhibits growth of the U-2OS cells,which could be a potential biomarker in the treatment of osteosarcoma.

关 键 词：miR-24-2 骨肉瘤 转染 增殖 

分 类 号：R738.7[医药卫生—肿瘤]