固相萃取-高效液相色谱法测定饮料中的罗丹明B  

Determination of rhodamine b in beverage by a solid phase extraction-high performance liquid chromatography

作　　者：许家胜[1] 张杰[1] 孙啸虎[1] 崔岩[1] 张帆[1] 袁德成 

机构地区：[1]渤海大学实验管理中心化学化工与食品安全学院 [2]95905部队

出　　处：《化学研究与应用》2014年第5期741-743,共3页Chemical Research and Application

摘　　要：建立使用固相萃取小柱净化样品,高效液相色谱测定饮料中的罗丹明B的方法。样品加磷酸混匀后过PXC（阳离子交换）固相萃取柱净化,氨水甲醇洗脱,洗脱液用氮气吹干后甲醇溶解进行高效液相色谱分析。流动相为甲醇-水（90∶10,V/V）,流速0．4 mL·min-1,外标法定量。罗丹明B在0．10-10．0μg·mL-1质量浓度范围内线性关系良好,相关系数R2为0．9996,最低检测限为0．015μg·mL-1。在空白样品中添加3个水平的标准品,回收率在88．79%~92．37%之间,相对标准偏差均小于4%（ n=6）。该方法简便、准确,适用于饮料中罗丹明B的检测。A novel method has been set up to determine the trace rhodamine B by high performance liquid chromatography. This method is used the solid phase extraction to purify the samples. The sample adding phosphoric acid mixing on a PXC （ cation ex-change） SPE column using ammonia and methanol as the eluate,and the eluate is evaporated to dryness and dissolved in methanol prior to HPLC analysis. The mobile phase is methanol-water（90∶10,V/V）at a flow rate of 0. 4 mL·min. Rhodamine B is quantified by external standard method. A good linear relationship between peak area and rhodamine B concentration is achieved in the range of 0. 10~10. 00 μg·mL-1（R2=0. 9996）,and the limit of detection（LOD）is 0. 015 μg·mL-1. The mean spike recovery rates of rhodamine B at three concentration levels are in the range of 88. 79% ~92. 37%,with relative standard deviation（ RSD） of less than 4%. The method is simple,accurate and suitable for the determination of rhodamine B in beverage.

关 键 词：液相色谱 罗丹明B 固相萃取 饮料 

分 类 号：O657.7[理学—分析化学]