mTORC1信号通路在花生四烯酸代谢产物致乳腺癌过程中的作用  

Role of mTORC1 in the process of promoting breast cancer induced by arachidonic acid metabolites

作　　者：刘淼[1] 张月[1] 何敏红[1] 周玲[1] 郑航[1] 

机构地区：[1]南方医科大学附属南方医院,广州510515

出　　处：《山东医药》2014年第14期22-24,共3页Shandong Medical Journal

基　　金：广东省自然科学基金资助项目(S201201008209);南方医科大学南方医院院长基金资助项目(2012B005)

摘　　要：目的探讨花生四烯酸(AA)代谢产物5-羟二十烷四烯酸(HETE)、12-HETE促进乳腺癌发生过程中雷帕霉素靶蛋白1(mTOR)信号通路的作用。方法将体外培养乳腺癌MCF-7细胞随机分为4组,对照组加入0.1%DMSO,HETE组加入5-HETE及12-HETE 15μmol/L,Rap组加入mTORC1抑制剂雷帕霉素(Rap)100μmol/L,HETE+Rap组加入Rap 100μmol/L及5-HETE+12-HETE 15μmol/L。CCK-8法检测各组细胞增殖率,免疫印迹法检测各组细胞mTORC1信号通路下游蛋白P-S6活性,细胞划痕实验检测各组细胞迁移能力。结果细胞增殖率HETE组高于对照组,HETE+Rap组低于HETE组(P均<0.05)。P-S6蛋白灰度值HETE组高于对照组,HETE+Rap组低于HETE组(P均<0.01)。MCF-7细胞迁移距离HETE组大于对照组(P<0.01)。HETE+Rap组小于HETE组(P<0.05)。结论 mTORC1信号通路参与了AA代谢产物乳腺癌发生的过程。Objective To investigate the role of mammalian target of rapamycin （ mTOR） complex 1 in the process of promoting breast cancer induced by arachidonic acid （AA） metabolites 5, 12-hydroxy-eicosatetraenoic acid （HETE）. Methods Breast cancer MCF-7 cells were cultured and then were divided into four groups .The control group was cultured with 0.1%DMSO.HETE group was cultured with 15 μmol/L 5-HETE and 12-HETE.Rap group was cultured with 100μmol/L mTORC1 inhibitor rapamycin （ Rap） .HETE ＋Rap group was cultured with Rap 100 μmol/L and 15 μmol/L 5-HETE ＋12-HETE.CCK-8 assay was used to detect the rate of cell proliferation .Western blotting was used to detect the activity change of the downstream protein mTORC 1 signaling pathway P-S6 protein.Wound healing was used to detect the migration activity.Results The cell proliferation rates of the control group , HETE group, Rap group, and HETE ＋Rap group was （0.85 ＆#177;0.01）, （1.20 ＆#177;0.13）, （0.53 ＆#177;0.08）, and （0.54 ＆#177;0.01）, respectively.The cell prolifera-tion rate of the HETE group was higher than that of the control group , whereas the cell proliferation rate of HETE ＋Rap group was lower than that of HETE group （all P＆lt;0.05）.The grey value of P-S6 of the control group, HETE group, Rap group, and HETE ＋Rap group was （0.85 ＆#177;0.01）, （1.20 ＆#177;0.13）, （0.53 ＆#177;0.08）, and （0.54 ＆#177;0.01）, re-spectively .The grey value of P-S6 of HETE group was higher than that of the control group , whereas the grey value of P-S6 of HETE＋Rap group lower than that of the HETE group （all P＆lt;0.01）.The MCF-7 cell migration distance in the HETE group was longer than that of the control group （P＆lt;0.01）, whereas MCF-7 cell migration distance in the HETE ＋Rap group was shorter than that of the HETE group （P＆lt;0.05）.Conclusion mTORC1 is involved in the process of promoting breast cancer induced by 5, 12-HETE.

关 键 词：羟二十烷四烯酸 雷帕霉素靶蛋白 花生四烯酸 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]